{"created":"2023-06-20T13:20:55.150645+00:00","id":989,"links":{},"metadata":{"_buckets":{"deposit":"06c7f6a6-d3fe-4968-bf01-ccae1c5821c7"},"_deposit":{"created_by":1,"id":"989","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"989"},"status":"published"},"_oai":{"id":"oai:ir.soken.ac.jp:00000989","sets":["2:430:20"]},"author_link":["10105","10104","10106"],"item_1_creator_2":{"attribute_name":"著者名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小池, 牧子"}],"nameIdentifiers":[{}]}]},"item_1_creator_3":{"attribute_name":"フリガナ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"コイケ, マキコ"}],"nameIdentifiers":[{}]}]},"item_1_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2003-09-30"}]},"item_1_degree_grantor_5":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"総合研究大学院大学"}]}]},"item_1_degree_name_6":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_1_description_12":{"attribute_name":"要旨","attribute_value_mlt":[{"subitem_description":"  The nucleocytoplasmic transport of macromolecules occurs through the nuclear pore complex (NPC).  Most transport events through the NPC are mediated by transport receptor molecules.  The localization of β-catenin to the nucleus is a crucial step in the transduction of the Wnt/Wingless signal.  Upon activation of the Wnt pathway, β-catenin accumulates in the nucleus.  Previous studies indicated that β-catenin could translocate on its own through the nuclear pores without the aid of small GTPase Ran, and shuttles between the cytoplasm and the nucleus.  The export of β-catenin also occurs in a Ran-independent manner.  Unlike receptor-mediated transport, β-catenin transport appears to use a rather unconventional mechanism, possibly through the direct interaction with NPC components.  In this study, I have analyzed the sequence requirement of β-catenin for its Ran-independent import and export in living mammalian cells and in the in vitro transport assay using semi-intact cells.  I confirmed that a β-catenin fragment containing both Armadillo repeats 10- 12 and the C-terminus possesses most strong nuclear import and export activity.  Further dissection of this fragment showed that C-terminus of β-catenin is most important for its import and export.  Moreover, I found that region required for β-catenin import and export overlaps, but they are not identical.  Competition studies using different transport receptors under different conditions indicated that interaction(s) required for β-catenin import and export differ at NPC.","subitem_description_type":"Other"}]},"item_1_description_18":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_1_description_7":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"総研大甲第723号","subitem_description_type":"Other"}]},"item_1_select_14":{"attribute_name":"所蔵","attribute_value_mlt":[{"subitem_select_item":"有"}]},"item_1_select_8":{"attribute_name":"研究科","attribute_value_mlt":[{"subitem_select_item":"生命科学研究科"}]},"item_1_select_9":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_select_item":"18 遺伝学専攻"}]},"item_1_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_value":"2003"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KOIKE, Makiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲723_要旨.pdf","filesize":[{"value":"162.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"要旨・審査要旨 / Abstract, Screening Result","url":"https://ir.soken.ac.jp/record/989/files/甲723_要旨.pdf"},"version_id":"e3f65cec-37b9-4523-8c54-32f2079e7472"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-17"}],"displaytype":"simple","filename":"甲723_本文.pdf","filesize":[{"value":"2.9 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://ir.soken.ac.jp/record/989/files/甲723_本文.pdf"},"version_id":"ed88166f-35fb-4c3d-a78e-25f87243687c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Sequence determination of region required  for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sequence determination of region required  for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage."},{"subitem_title":"Sequence determination of region required for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage.","subitem_title_language":"en"}]},"item_type_id":"1","owner":"1","path":["20"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"989","relation_version_is_last":true,"title":["Sequence determination of region required  for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage."],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-06-20T14:40:34.595014+00:00"}