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Estimation of statistical binding properties of ligand population during in vitro selection based on population dynamics theory.
https://ir.soken.ac.jp/records/5328
https://ir.soken.ac.jp/records/532859820c7e-5c24-4d49-9510-dc1eb10c12fd
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2016-04-22 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Estimation of statistical binding properties of ligand population during in vitro selection based on population dynamics theory. | |||||||||||
| タイトル | ||||||||||||
| タイトル | Estimation of statistical binding properties of ligand population during in vitro selection based on population dynamics theory. | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Computer Simulation | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | In Vitro Techniques | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Ligands | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Models, Chemical | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Peptides | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Thermodynamics | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | metadata only access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||
| 著者 |
AITA, Takuyo
× AITA, Takuyo
× NISHIGAKI, Koichi
× HUSIMI, Yuzuru
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| 著者注記 | ||||||||||||
| 値 | Yuzuru, Husimi : Innovative Research Organization, Saitama University | |||||||||||
| 著者注記 | ||||||||||||
| 値 | ※ The Graduate University for Advanced Studies *(professor) 2014.8- | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | During in vitro selection process, it is very valuable to monitor the binding properties of the ligand population in real time, particularly the population average of the association constant in the population. If this monitoring can be realized, the selection process can be controlled in a rational way. In this paper, we present a simple method to estimate the binding properties of the ligand population during in vitro selection. The framework of the method is as follows. First, the number of all the collected ligand molecules, which are eluted after incubation and washing, is measured. Ideally, this number corresponds to the number of all the ligand molecules bound with the target-receptor or other materials in a test tube. This measurement is performed through several successive rounds of selection. Second, the measured numbers of molecules are subjected to a theoretical analysis, based on the mathematical theory of population dynamics in the selection process. Then, we can estimate the probability density of the binding free energy in the ligand population. The validity of our method was confirmed by several computer simulations based on a physicochemical model. | |||||||||||
| 論文注記 | ||||||||||||
| 値 | Available online 15 November 2013 | |||||||||||
| 書誌情報 |
Mathematical biosciences en : Mathematical biosciences 巻 247, p. 59-68, 発行日 2014-01 |
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| 雑誌情報 | ||||||||||||
| 値 | Publication Start Year : 1967 | |||||||||||
| 雑誌情報 | ||||||||||||
| 値 | Frequency : Monthly | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | American Elsevier / New York | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0025-5564 | |||||||||||
| 書誌レコードID (NCID) | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA11534002 | |||||||||||
| PubMed番号 | ||||||||||||
| 識別子タイプ | PMID | |||||||||||
| 関連識別子 | 24239675 | |||||||||||
| DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | http://dx.doi.org/10.1016/j.mbs.2013.10.012 | |||||||||||
| 関連名称 | 10.1016/j.mbs.2013.10.012 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | © 2013 Elsevier Inc. All rights reserved. | |||||||||||
| 関連サイト | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | http://www.sciencedirect.com/science/journal/00255564 | |||||||||||
| 関連名称 | Mathematical Biosciences ＜ELSEVIER ScienceDirect＞ | |||||||||||
