{"_buckets": {"deposit": "59ce15d9-25a0-466f-a356-67c29ae7d3df"}, "_deposit": {"created_by": 1, "id": "1024", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1024"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00001024", "sets": ["20"]}, "author_link": ["0", "0", "0"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-09-29", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "藤井, 智明"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "フジイ, トモアキ"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2006-09-29"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "2006522", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u003ci\u003eGsdm/GSDM\u003c/i\u003e is a novel gene family consisting of structurally-related seven mouse genes and four human genes. A member of this family，\u003ci\u003eGsdma3\u003c/i\u003e，is known to be the causative gene of a mouse skin mutant \u003ci\u003eRim3\u003c/i\u003e，which exhibits epidermal hyperplasia，hyperkeratosis and abnormal hair development. All members of the \u003ci\u003eGsdm/GSDM\u003c/i\u003e family are expressed predominantly in epithelial cells of various different tissues including skin and gastrointestinal tract. Thus far，expression patterns of \u003ci\u003eGsdma genes\u003c/i\u003e clustering in the mouse chromosome 11 have been well analyzed，and it is revealed that they are expressed in skin and upper gastrointestinal tracts，such as esophagus and stomach，in a tissue specific manner. The phenotypes of \u003ci\u003eRim3\u003c/i\u003e and other mutants of \u003ci\u003eGsdma3\u003c/i\u003e have been well characterized．The results of these studies suggested that function of the \u003ci\u003eGsdm/GSDM\u003c/i\u003e gene family is involved in regulation of proliferation and differentiation of epithelial cells. \u003cbr /\u003e　　　In mouse，other members of the \u003ci\u003eGsdm/GSDM\u003c/i\u003e family，\u003ci\u003eGsdmc\u003c/i\u003e and \u003ci\u003eGsdmd\u003c/i\u003e，are known to be expressed in intestinal tracts, small intestine and colon, but information about their genome structures and fine expression patterns is very limited. Moreover, functional analysis of the \u003ci\u003eGsdmc\u003c/i\u003e and \u003ci\u003eGsdmc\u003c/i\u003e genes has been hampered by absence of available mutants.\u003cbr /\u003e　　　In this study, first I carried our molecular cloning of a new \u003ci\u003eGsdmc\u003c/i\u003e-related gene, and analyzed fine genome structures of the \u003ci\u003eGsdmc\u003c/i\u003eand \u003ci\u003eGsdmd\u003c/i\u003e genes primarily by intensive search for public genome databases. As a consequence, I found that four \u003ci\u003eGsdmc\u003c/i\u003egenes are clustering in the mouse chromosome 15, and \u003ci\u003eGsdmd\u003c/i\u003e is linkcd to the \u003ci\u003eGsdmc\u003c/i\u003e cluster. Second, I conducted extensive analysis of expression profiling of the \u003ci\u003eGsdmc\u003c/i\u003eand \u003ci\u003eGsdmd\u003c/i\u003egenes by Northern blot, quantitative PCR and \u003ci\u003ein situ\u003c/i\u003e hybridization. The results clearly showed that the expression patterns of these genes are highly tissue specific. Moreover，Position restricted expression of each gene is observed . It was also notable that all genes are expressed in differentiated cells but not in proliferating cells and stem cells, as is the case with the \u003ci\u003eGsdma\u003c/i\u003ecluster genes. \u003cbr /\u003e　　　Finally, I generated \u003ci\u003eGsdmd\u003c/i\u003e-null mutant mice to elucidate function of this gene. Surprisingly, the mutant mice showed no marked phenotype in the external appearance, histology of the small intestine and the colon, and cell differentiation and proliferation in the intestinal tract. However, I found that mortality of the mutant homozygotes is elevated after 5 months of age. Furthermore, mutant mice exposed to \u0026gamma;-irradiation revealed to have defect in cell proliferation and tissue recovering in the intestinal tract after injury. This result demonstrated that \u003ci\u003eGsdmd\u003c/i\u003eplays a role in homeostasis rather than development and morphogenesis of intestinal tract. All results of the study showed that functions of the members of the \u003ci\u003eGsdm\u003c/i\u003e family are not simple, but might be rather diverse depending on tissues. Taking account of the strictly restricted and differential expression patterns of each member of the \u003ci\u003eGsdm\u003c/i\u003egene family, may play differential functions depending on tissues that express specific member(s) of the \u003ci\u003eGsdm\u003c/i\u003e gene family.", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第1002号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_16": {"attribute_name": "複写", "attribute_value_mlt": [{"subitem_select_item": "複写不可"}]}, "item_1_select_17": {"attribute_name": "公開状況", "attribute_value_mlt": [{"subitem_select_item": "要旨のみ公開"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "18 遺伝学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2006"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "FUJII, Tomoaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲1002_要旨.pdf", "filesize": [{"value": "233.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 233100.0, "url": {"label": "要旨・審査要旨", "url": "https://ir.soken.ac.jp/record/1024/files/甲1002_要旨.pdf"}, "version_id": "3145e863-ea01-4e82-a0f6-eb1a083c387b"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Expression profiling and functional analysis of Gasdermin (Gsdm) family genes in mouse intestinal tract", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Expression profiling and functional analysis of Gasdermin (Gsdm) family genes in mouse intestinal tract"}, {"subitem_title": "Expression profiling and functional analysis of Gasdermin (Gsdm) family genes in mouse intestinal tract", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["20"], "permalink_uri": "https://ir.soken.ac.jp/records/1024", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1024", "relation": {}, "relation_version_is_last": true, "title": ["Expression profiling and functional analysis of Gasdermin (Gsdm) family genes in mouse intestinal tract"], "weko_shared_id": 1}