| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2010-02-22 |
| タイトル |
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|
タイトル |
Proper initiation of chromosomal DNA replication requires the Sld3-Sld7 complex in budding yeast |
| タイトル |
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|
タイトル |
Proper initiation of chromosomal DNA replication requires the Sld3-Sld7 complex in budding yeast |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
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資源タイプ |
thesis |
| 著者名 |
田中, 太門
|
| フリガナ |
タナカ, タモン
|
| 著者 |
TANAKA, Tamon
|
| 学位授与機関 |
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学位授与機関名 |
総合研究大学院大学 |
| 学位名 |
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学位名 |
博士（理学） |
| 学位記番号 |
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内容記述タイプ |
Other |
|
内容記述 |
総研大甲第1160号 |
| 研究科 |
|
|
値 |
生命科学研究科 |
| 専攻 |
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|
値 |
18 遺伝学専攻 |
| 学位授与年月日 |
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学位授与年月日 |
2008-03-19 |
| 学位授与年度 |
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|
値 |
2007 |
| 要旨 |
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内容記述タイプ |
Other |
|
内容記述 |
Eukaryotic chromosomal DNA replication initiates from replication origins only <br />once per cell cycle. Many proteins assemble on origins, form replication machinery, and <br />then dissociate from origins to start DNA synthesis. However, the mechanism governing <br />these processes has not been well elucidated. Here, l show that the Sld7 protein <br />participates in the dissociation process between replication machinery and origins. <i>SLD7</i> (<u>S</u>ynthetic <u>L</u>ethality with <u>D</u>pb 11-24 <u>7</u>), a newly identified gene in a synthetic lethal <br />screening, is not essential for cell growth but required for several cellular processes, such as an efficient chromosomal DNA replication. The Sld7 protein always forms a complex with the Sld3 protein, which functions for the initiation of chromosomal DNA replication, to enhance the Sld3 function. Thus, high-copy <i>SLD3</i> gene compensates for the lack of Sld7, and high-copy <i>SLD7</i> gene suppresses the sld3-6 mutation that reduces the complex formation between Sld3 and Sld7 proteins. This Sld3-Sld7 complex associates with replication origins in Gl -phase and dissociates gradualty from them when replication initiates. Sld3 protein associates with and then dissociates from origins in the absence of SId7. The Mcm7 protein, a component of replication machinery, associates with origins and then with neighboring regions of origins as it moves with replication forks. In the absence of Sld7 protein, although Mcm7 associates with origins as efficient as in WT cells, its association with neighboring region is significantly reduced. Therefore, the Sld7 protein seems to help the efficient displacement of replication machinery from origins. <br /> |
| 所蔵 |
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|
値 |
有 |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
要旨・審査要旨 (344.5 kB)
