{"_buckets": {"deposit": "404b6f98-6aa4-4232-b8bc-d637f9129342"}, "_deposit": {"created_by": 1, "id": "1043", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1043"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00001043", "sets": ["20"]}, "author_link": ["0", "0", "0"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-03-19", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "齋藤, 憲二"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "サイトウ, ケンジ"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2008-03-19"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "2008054", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "　　Continuation of spermatogenic process throughout life relies on a proper\u003cbr /\u003eregulation of self-renewal and differentiation of spermatogonial stem cells which is\u003cbr /\u003epresent in a special cellular organization called a niche. In spite of biological\u003cbr /\u003esignificance of the spermatogonial stem cells, little is known about their behavior and\u003cbr /\u003eproperties because of a lack of model system to approach the stem cell maintenance\u003cbr /\u003e and differentiation in several organisms. \u003cbr /\u003e　　In this study, I show the presence of subpopulations in zebrafish type A\u003cbr /\u003espermatogonia which is classically considered homogeneous pepulation varying in\u003cbr /\u003esize, location pattern and cell cycle phase. Morphological analysis showed two types\u003cbr /\u003eof type A sperrnatogonia that are focalized distant from basement membranes (termed\u003cbr /\u003etype A\u003csmall\u003edt\u003c/small\u003e) and close to basement membranes (A\u003csmall\u003ecl\u003c/small\u003e). The thymidine analogue \u003cbr /\u003e5-bromo-2\u0027-deoxyuridine (BrdU) incorporation experiments showed that duration of \u003cbr /\u003ecell cycle of the type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia was longer than that of the.type A\u003csmall\u003edt\u003c/small\u003e \u003cbr /\u003espermatogonia. Tracing label-retaining cells (LRCs), after 4 weeks of chase, I \u003cbr /\u003eobserved occasionally BrdU-positive type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia. Immunohistochemical\u003cbr /\u003e analysis using antibody against zebrafish homologue of Synthetic lethal mutant of \u003cbr /\u003edpb11-1 (zSld5) which is able to distinguish between the guiescent cells and the \u003cbr /\u003eproliferation cells showed that type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia could be divided into two\u003cbr /\u003esubpopulations. One population is zSld5-positive type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia and the other \u003cbr /\u003eis zSld5-negative type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia In addition, many LRCs are contained in a\u003cbr /\u003elatter population. All the results suggest that type zSld5-negative type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia \u003cbr /\u003eare most undifferentiated type A spermatogonia. \u003cbr /\u003e　　It is generally known that spermatogonial stem cells have ability to restore \u003cbr /\u003espermatogenesis when a testis was damaged by a drug or irradiation. To elucidate the\u003cbr /\u003echaracterization of type A spermatogonia, I examined the effect of cytotoxic reagent \u003cbr /\u003ebusulfan on male germ cells. As a result, only type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia survived after \u003cbr /\u003etreatment of busulfan, and then recovered spermatogenesis. The morphological \u003cbr /\u003eobservation suggests that type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia are the most resistant cell population \u003cbr /\u003eagainst busulfan in the zebrafish testis. Detailed analysis revealed that the number of \u003cbr /\u003ezSld5-positive type type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia increased transiently after busulfan treatment, while the number of type zSld5-negative type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia relatively stabilized. These results suggest that both zSld5-negative and zSld5-positive type A\u003csmall\u003ecl\u003c/small\u003e spermatogonia would posses a feature of spermatogonial stem cells, like a actual stem cell and a potential stem cell, respectively. \u003cbr /\u003e　　Furthermore, I developed the \u003ci\u003ein vitro\u003c/i\u003e culture condition for supporting the maintenance and proliferation of type A spermatogonia by the use of Sertoli feeder ZtA6-6 cells for 1 month or so. \u003cbr /\u003e　　This study represents the first step towards further understanding the character of spermatogonial stem cells and molecular mechanisms to maintain the undifferentaition status in teleosts.", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第1161号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_16": {"attribute_name": "複写", "attribute_value_mlt": [{"subitem_select_item": "印刷物から複写可"}]}, "item_1_select_17": {"attribute_name": "公開状況", "attribute_value_mlt": [{"subitem_select_item": "全文公開可"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "18 遺伝学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2007"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "SAITO, Kenji", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲1161_要旨.pdf", "filesize": [{"value": "234.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 234100.0, "url": {"label": "要旨・審査要旨", "url": "https://ir.soken.ac.jp/record/1043/files/甲1161_要旨.pdf"}, "version_id": "37fb3810-fe45-4aa8-8f70-e89ccdc11674"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "甲1161_本文.pdf", "filesize": [{"value": "5.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 5200000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/1043/files/甲1161_本文.pdf"}, "version_id": "857c3abf-e201-4010-8ecf-1df9cae7ee5a"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Characterization of type A spermatogonia and development of the culture system in zebrafish", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Characterization of type A spermatogonia and development of the culture system in zebrafish"}, {"subitem_title": "Characterization of type A spermatogonia and development of the culture system in zebrafish", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["20"], "permalink_uri": "https://ir.soken.ac.jp/records/1043", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1043", "relation": {}, "relation_version_is_last": true, "title": ["Characterization of type A spermatogonia and development of the culture system in zebrafish"], "weko_shared_id": 1}