{"_buckets": {"deposit": "9cd9347d-a021-4949-b889-c81c7a5c0b2a"}, "_deposit": {"created_by": 1, "id": "1070", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1070"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00001070", "sets": ["21"]}, "author_link": ["0", "0", "0"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-09-30", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "CHOW, PAKHONG Jeremy"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "チョウ, パク ホンジェレミー"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2008-09-30"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "2008523", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "Protein tyrosine phosphatase receptor type Z (Ptprz, also known as PTP\u0026zeta; or RPTPβ) is\u003cbr /\u003epreferentially expressed in the brain as a major chondroitin sulfate proteoglycan. \u003cbr /\u003eThree splicing variants, two receptor-type isoforms and one secretory isoform, are\u003cbr /\u003eknown. 　　Ptprz interacts with the PSD95 family through its intracellular\u003cbr /\u003ecarboxyl-terminal PDZ-binding motif in the postsynaptic density of the adult brain. \u003cbr /\u003e\u003ci\u003ePtprz\u003e\u003c/i\u003e-deficient mice display impairments in spatial and contextual learning. 　Here, I\u003cbr /\u003eshow that the extracellular region of the receptor isoforms of Ptprz are cleaved by\u003cbr /\u003emetalloproteinases including ADAM-17 (TACE) and subsequently the\u003cbr /\u003emembrane-tethered fragment is cleaved by presenilin/\u0026gamma;-secretase, releasing its\u003cbr /\u003eintracellular region into the cytoplasm: 　Noteworthily, the intracellular fragment of\u003cbr /\u003ePtprz shows nuclear localization.  Administration of GM6001, an inhibitor of\u003cbr /\u003emetalloproteinases, to mice demonstrated the metalloproteinase-mediated cleavage of\u003cbr /\u003ePtprz under physiological conditions. Furthermore, I identified the cleavage sites in\u003cbr /\u003ethe extracellular juxtamembrane region of Ptprz by TACE and MMP-9. This is the\u003cbr /\u003efirst evidence of the metalloproteinase-mediated processing of an RPTP in the central\u003cbr /\u003enervous system. \u003cbr /\u003e　I also identified the proteolytic processing of Ptprz by plasmin in the adult mouse\u003cbr /\u003ebrain, which is markedly enhanced after kainate-induced seizures. We estimated the\u003cbr /\u003ecleavage sites in the extracellular region of Ptprz based on cell-based assays and \u003ci\u003ein vitro\u003c/i\u003edigestion experiments with recombinant proteins. The findings indicate that Ptprz is a\u003cbr /\u003ephysiological target for activity-dependent proteolytic processing by the tPA/plasmin\u003cbr /\u003esystem, and suggest that the proteolytic fragments are involved in the structural and\u003cbr /\u003efunctional processes of the synapses during learning and memory. \u003cbr /\u003e", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第1204号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "19 基礎生物学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2008"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "CHOW, PAKHONG Jeremy", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲1204_要旨.pdf", "filesize": [{"value": "186.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 186000.0, "url": {"label": "要旨・審査要旨", "url": "https://ir.soken.ac.jp/record/1070/files/甲1204_要旨.pdf"}, "version_id": "e5604c9c-3cc2-4d00-84cd-5dc1da540274"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "甲1204_本文.pdf", "filesize": [{"value": "10.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 10000000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/1070/files/甲1204_本文.pdf"}, "version_id": "ef6f1743-1cbf-4a1e-a520-480529385c2c"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Proteolytic processing of protein tyrosine phosphatase receptor type Z in the CNS", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Proteolytic processing of protein tyrosine phosphatase receptor type Z in the CNS"}, {"subitem_title": "Proteolytic processing of protein tyrosine phosphatase receptor type Z in the CNS", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["21"], "permalink_uri": "https://ir.soken.ac.jp/records/1070", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1070", "relation": {}, "relation_version_is_last": true, "title": ["Proteolytic processing of protein tyrosine phosphatase receptor type Z in the CNS"], "weko_shared_id": 1}