| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2010-02-22 |
| タイトル |
|
|
タイトル |
Application of Zernike Phase Plate to Transmission Electron Microscopy |
| タイトル |
|
|
タイトル |
Application of Zernike Phase Plate to Transmission Electron Microscopy |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 著者名 |
Danev, Radostin Stoyanov
|
| フリガナ |
ダネブ, ラドスティン ストヤノフ
|
| 著者 |
Danev, Radostin Stoyanov
|
| 学位授与機関 |
|
|
|
学位授与機関名 |
総合研究大学院大学 |
| 学位名 |
|
|
学位名 |
博士（理学） |
| 学位記番号 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
総研大甲第562号 |
| 研究科 |
|
|
値 |
生命科学研究科 |
| 専攻 |
|
|
値 |
20 生理科学専攻 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2001-09-28 |
| 学位授与年度 |
|
|
値 |
2001 |
| 要旨 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
The possibility of implementing a Zernike phase plate in a transmission electron microscope is investigated both theoretically and experimentally. The phase-retarding plate in the form of thin film with a hole in the center is positioned in the back-focal plane of the objective lens. The experiments showed that the phase plate functions as predicted, producing a cosine-type phase contrast transfer function. Images of negatively stained horse spleen ferritin were highly improved in the contrast and the image-modulation, compared to those acquired without the phase plate. Electrostatic charging and related difficulties were encountered during the phase plate experiments.<br />The radial distribution of the beam-induced charge in thin films was investigated using the contrast transfer properties of the transmission electron microscope. The phase shift due to charging was measured as the phase difference between the contrast transfer functions of two photos taken with and without film at the back focal plane. Solving the inverse Laplace problem with this input data recovers the charge density of the measured film. The surface charge density reaches quasi-equilibrium state after the first 30 min of the electron beam pre-irradiation. An explanation of the qualitative behavior of the charge density, based on the contamination diffusion theory, is proposed.<br />The first experimental realization of the complex reconstruction scheme is presented. Two images taken with and without Zernike phase plate are used for the extraction of both the phase and the amplitude of the object wave. New “off- plane” application scheme for the phase plate was developed, to overcome various practical problems. Aberration-corrected phase images of negatively stained horse spleen ferritin and tobacco mosaic virus samples are free from image distortion and show extraordinary high contrast compared to that obtained with conventional TEM. |
| 所蔵 |
|
|
値 |
有 |
要旨・審査要旨 / Abstract, Screening Result (198.5 kB)
