| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2010-02-22 |
| タイトル |
|
|
タイトル |
The Origin of Oligodendrocyte in Mouse Forebrain |
| タイトル |
|
|
タイトル |
The Origin of Oligodendrocyte in Mouse Forebrain |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 著者名 |
中平, 英子
|
| フリガナ |
ナカヒラ, エイコ
|
| 著者 |
NAKAHIRA, Eiko
|
| 学位授与機関 |
|
|
|
学位授与機関名 |
総合研究大学院大学 |
| 学位名 |
|
|
学位名 |
博士（理学） |
| 学位記番号 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
総研大甲第696号 |
| 研究科 |
|
|
値 |
生命科学研究科 |
| 専攻 |
|
|
値 |
20 生理科学専攻 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2003-03-24 |
| 学位授与年度 |
|
|
値 |
2002 |
| 要旨 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Oligodendrocytes (OLs) are myelin-forming cells in the central nervous system. Although the function of OLs is well studied in the adult nervous system, little is known about the embryonic origin of this cell type, especially in the cerebral cortex. There were no direct methods to identify the origin of OLs, because OL progenitors are highly migratory and mitotic. In this study, I established a permanent cell labeling system, named CRE-EP system assisted with in utero DNA electroporation and a Cre reporter strain of mouse. Using this new system, I labeled the cells surrounding the lateral ventricle of the forebrain in ventral or dorsal specific manner, and traced their cell fate even into the adult ages. Many OLs were detected in the adult cerebral cortex originated from the subpallial ventricular zone, but was no OLs of pallium origin, indicating that most, if not all, myelinating OLs in the cortex are originated from the ventral forebrain. There were two distinct populations of migrating OL lineage cells observed in the cortex. The first wave of OL progenitor migration was observed in the intermediate zone (IZ) at El6. These cells expressed platelet derived growth factor receptor α subunit (PDGFRα) at as a marker, arose from the ventral neuroepithelium early embryonic days, and tangentially migrated into the cortex through the IZ rather than the subventricular zone (SVZ) or ventricular zone. I also observed Olig2<SUP>+</SUP> / PDGFRα<SUP>-</SUP> cellsinthe cortical SVZat E16. These cells seems to turn out to be PDGFRα<SUP>+</SUP> within the SVZ by E18, and radially migrate towards the cortical plate, so be named the "second wave" of migration. |
| 所蔵 |
|
|
値 |
有 |
要旨・審査要旨 / Abstract, Screening Result (192.1 kB)
