| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2010-02-22 |
| タイトル |
|
|
タイトル |
Cystatin C Regulates Astrocyte Development |
| タイトル |
|
|
タイトル |
Cystatin C Regulates Astrocyte Development |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 著者名 |
長谷川, 明子
|
| フリガナ |
ハセガワ, アキコ
|
| 著者 |
HASEGAWA, Akiko
|
| 学位授与機関 |
|
|
|
学位授与機関名 |
総合研究大学院大学 |
| 学位名 |
|
|
学位名 |
博士（理学） |
| 学位記番号 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
総研大甲第697号 |
| 研究科 |
|
|
値 |
生命科学研究科 |
| 専攻 |
|
|
値 |
20 生理科学専攻 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2003-03-24 |
| 学位授与年度 |
|
|
値 |
2002 |
| 要旨 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
She studied the effects of cystatin C (CysC) on astrocyte development. CysC is an endogenous inhibitor of cystein protease, known to be produced by mature astrocytes in the adult brain. Previously, CysC was isolated as a factor regulating astrocyte development through a newly developed expression cloning system using glial fibrillary acidic protein (GFAP)-promoter activity as an indicator. An in situ hybridization study showed that the CysC gene started to be expressed in astrocyte progenitor cells at embryonic day 16 (E16) in mouse cortex, when gliogenesis is initiated following the generation of neurons in the cortex.<br /> CysC is a secreted protein, thus it is possible that CysC produced by the astrocyte progenitors acts as an epigenetic factor to promote gliogenesis in the developing cortex in an autocrine/paracrine manner. Recently, it was reported that CysC is a cofactor of basic fibroblast growth factor-2 (FGF-2) and works to maintain and proliferate the neural stem cells (NSC) as an autocrine/paracrine factor in vitro (Taupin et al., 2000). She showed that the protease inhibitory activity, in the conditioned medium (CM) from primary cultured brain cells, increased day by day. She found one anti-CysC antibody that blocked the inhibitory activity of the CM, indicating that the primary cultured cells released CysC into the medium. She also demonstrated that CysC added to the culture medium increased the number and the process length of GFAP positive cells in the primary cultured brain cells. On the other hand, addition of the anti-CysC antibody decreased those. Another cystein protease inhibitor, E64, increased the process length of GFAP positive cells but did not caused an increase the number of GFAP positive cells in the primary cultured brain cell. These results showed that CysC can modify astrocyte development in two ways: one is to increase the number of astrocytes, which is independent of its protease inhibitor activity and the other to increase the process length of astrocytes, which is dependent on its protease inhibitor activity. |
| 所蔵 |
|
|
値 |
有 |
要旨・審査要旨 / Abstract, Screening Result (199.9 kB)
