WEKO3
アイテム
DEVELOPMENT OF THE HIGH-SPEED VIDEO-OCULOGRAPHIC SYSTEM AND ITS APPLICATION TO THE STUDY ON THE SACCADIC EYE MOVEMENTS IN MICE
https://ir.soken.ac.jp/records/1141
https://ir.soken.ac.jp/records/11415963275e-4a64-4c9a-8e7a-394ec0191b86
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
要旨・審査要旨 / Abstract, Screening Result (247.1 kB)
|
||
|
本文 (7.3 MB)
|
| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-02-22 | |||||
| タイトル | ||||||
| タイトル | DEVELOPMENT OF THE HIGH-SPEED VIDEO-OCULOGRAPHIC SYSTEM AND ITS APPLICATION TO THE STUDY ON THE SACCADIC EYE MOVEMENTS IN MICE | |||||
| タイトル | ||||||
| タイトル | DEVELOPMENT OF THE HIGH-SPEED VIDEO-OCULOGRAPHIC SYSTEM AND ITS APPLICATION TO THE STUDY ON THE SACCADIC EYE MOVEMENTS IN MICE | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 著者名 |
坂谷, 智也
× 坂谷, 智也 |
|||||
| フリガナ |
サカタニ, トモヤ
× サカタニ, トモヤ |
|||||
| 著者 |
SAKATANI, Tomoya
× SAKATANI, Tomoya |
|||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 総合研究大学院大学 | |||||
| 学位名 | ||||||
| 学位名 | 博士（理学） | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 総研大甲第777号 | |||||
| 研究科 | ||||||
| 値 | 生命科学研究科 | |||||
| 専攻 | ||||||
| 値 | 20 生理科学専攻 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2004-03-24 | |||||
| 学位授与年度 | ||||||
| 値 | 2003 | |||||
| 要旨 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Due to the increasing use of transgenic and knockout mice in neuroscience research, it is urgently necessary to develop the technique for quantitative measurement of behaviors in mice. The video-oculography is often used to measure the eye movements of mice, but due to its limitation in temporal resolution, its use was limited to analysis of slow eye movements such as vestibuloocular reflex and optokinetic responses. As a result, rapid eye movements such as saccades have not been properly analyzed in mice as yet. To overcome such difficulties, I have newly developed a video-oculography system with high temporal resolution (240Hz) by combining high speed CCD camera with image processing software written in LabVIEW run on IBM-PC. By this recording system, the dynamic characteristics of the rapid eye movements (saccades) in alert, head-fixed mice(C57BL/6 strain) were investigated.<br /> Mice spontaneously made rapid eye movements in the dark at a frequency of 3.2 /min with mean amplitude of 9 degrees mainly in horizontal direction. The peak velocity of saccades increased almost linearly against the saccadic amplitude with slope of 54 / sec, whereas the saccadic duration saturated at the level of 60 msec against larger saccades.<br /> These saccade-like rapid eye movements could be induced by the electrical stimulations of the intermediate and deep layers of the SC in a head-fixed condition. Suprathreshold current stimulus evoked mainly contraversive saccades having a roughly constant vector depending on the stimulus sites in the SC. The amplitude and the direction of evoked saccades highly depended on the initial eye position in the orbit as well as on the stimulus sites in the SC, and evoked saccades were goal-directed in mice, suggesting that eye position signals might strongly affect the dynamics of saccades in this species. Though, most sites in the SC yielded contraversive characteristic saccades, from the antero-medial region, where the visual representation of the superficial layer shared the same location in the visual field with the opposite SC, ipsiversive saccades could be induced.<br /> For further investigation of the mechanisms underlying saccades, especially molecular mechanisms of saccade generator circuitry, the dynamic characteristics of both the spontaneous and the electrically induced saccades were examined in mice lacking a GABA-synthesizing enzyme, glutamic acid decarboxylase (GAD) 65, and a functional role of the inhibitory neurotransmitter gamma-amino butyric acid(GABA) in the saccadic system was analyzed. The peak velocity of the saccadic eye movements was significantly higher and the amplitude of saccades tended to be smaller in GAD65 knockout mice than those in wild-type animals. Moreover, significant increase in fluctuations was observed in the eye position after the termination of saccades in the GAD65 knockout mice. Prolonged electrical stimulation of the SC often evoked oscillation-like eye movements after saccades in the mutant mice. Computer simulations based on the control system model with a negative feedback loop could attribute these experimental results to the increase in the efficacy of feedback loop in the saccade generator circuitry in the downstream of the SC in GAD65 knockout mice. These results support the hypothesis that the neural system underlying the saccade generation consists of a feedback loop, and suggest that GABA plays an important role in stabilizing the saccade control system in the downstream of the SC.<br /> The results presented in this study show that the newly developed video-oculographic system provides a powerful tool for analyzing dynamic characteristics of saccadic eye movements in mice. Furthermore, this study will provide a fundamental knowledge to study molecular basis of the oculomotor and, moreover, cognitive functions by using various lines of mutant mice. | |||||
| 所蔵 | ||||||
| 値 | 有 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
