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Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL)
https://ir.soken.ac.jp/records/1165
https://ir.soken.ac.jp/records/11659b1a02af-43c1-4fe9-8be4-c6a3b9bc4539
| 名前 / ファイル | ライセンス | アクション |
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要旨・審査要旨 (168.8 kB)
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本文 (18.0 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2010-02-22 | |||||
| タイトル | ||||||
| タイトル | Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL) | |||||
| タイトル | ||||||
| タイトル | Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL) | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 著者名 |
春日井, 雄
× 春日井, 雄 |
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| フリガナ |
カスガイ, ユウ
× カスガイ, ユウ |
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| 著者 |
KASUGAI, Yu
× KASUGAI, Yu |
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| 学位授与機関 | ||||||
| 学位授与機関名 | 総合研究大学院大学 | |||||
| 学位名 | ||||||
| 学位名 | 博士（理学） | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 総研大甲第963号 | |||||
| 研究科 | ||||||
| 値 | 生命科学研究科 | |||||
| 専攻 | ||||||
| 値 | 20 生理科学専攻 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2006-03-24 | |||||
| 学位授与年度 | ||||||
| 値 | 2005 | |||||
| 要旨 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Pyramidal cells in he hippocampal CA1 area express at least 14 subunits of the<br />GABA-A receptor, and receive GABAergic input from interneurons, which make<br />synapses on the soma,dendrites and the axon initial segment(AIS).Here,they used<br />the SDS-digested freeze-fracture replica labelling method to visualize the cell surface<br />distribution of the alpha1,alpha2,and beta2/3 subunits quantitatively on distinct<br />subcellular compartments of pyramidal cells. Immunogold particles for these subunits<br />were accumulated over clusters of intramembrane particles(IMP) on the protoplasmic<br />face(P-face) of the plasma membrane, indicating that many IMP clusters of a certain<br />size represent GABAergic synapses.<br />The sizes of labelled synapses were not different on the somata and the main apical<br />dendritic trunks.However,the synaptic areas on AISs were significantly smaller than<br />those on the soma and apical dendrites. Axon initial segments were identified by<br />immunogold Labelling for the Nav1.6 subunit of sodium channels. The synaptic<br />receptor labelling densities for GABA-A receptor subunits were not significantly<br />different on the tested areas of the cells. A high proportion of receptor labelling was on<br />the extrasynaptic membrane of the pyramidal cell soma and dendrites. To examine the<br />co-localization of different GABA-A receptor subunits in single synapses on the soma,<br />double labelling was performed with antibodies raised in different species. These<br />experiments showed that the majority of synapses contains alpha1,alpha2,and beta2/3<br />subunits. Future experiments aim at testing whether distinct synapese populations<br />differing in the relative proportions of different subunits can be detected with freeze<br />fracture replica immunogold labelling. | |||||
| 所蔵 | ||||||
| 値 | 有 | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
