WEKO3
アイテム
{"_buckets": {"deposit": "a7d1bc4f-a906-4be4-824a-7ed98d66e6b0"}, "_deposit": {"created_by": 1, "id": "1165", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1165"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00001165", "sets": ["22"]}, "author_link": ["0", "0", "0"], "item_1_biblio_info_21": {"attribute_name": "書誌情報(ソート用)", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-03-24", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "春日井, 雄"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "カスガイ, ユウ"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2006-03-24"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(理学)"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "2006050", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "Pyramidal cells in he hippocampal CA1 area express at least 14 subunits of the\u003cbr /\u003eGABA-A receptor, and receive GABAergic input from interneurons, which make\u003cbr /\u003esynapses on the soma,dendrites and the axon initial segment(AIS).Here,they used\u003cbr /\u003ethe SDS-digested freeze-fracture replica labelling method to visualize the cell surface\u003cbr /\u003edistribution of the alpha1,alpha2,and beta2/3 subunits quantitatively on distinct\u003cbr /\u003esubcellular compartments of pyramidal cells. Immunogold particles for these subunits\u003cbr /\u003ewere accumulated over clusters of intramembrane particles(IMP) on the protoplasmic\u003cbr /\u003eface(P-face) of the plasma membrane, indicating that many IMP clusters of a certain\u003cbr /\u003esize represent GABAergic synapses.\u003cbr /\u003eThe sizes of labelled synapses were not different on the somata and the main apical\u003cbr /\u003edendritic trunks.However,the synaptic areas on AISs were significantly smaller than\u003cbr /\u003ethose on the soma and apical dendrites. Axon initial segments were identified by\u003cbr /\u003eimmunogold Labelling for the Nav1.6 subunit of sodium channels. The synaptic\u003cbr /\u003ereceptor labelling densities for GABA-A receptor subunits were not significantly\u003cbr /\u003edifferent on the tested areas of the cells. A high proportion of receptor labelling was on\u003cbr /\u003ethe extrasynaptic membrane of the pyramidal cell soma and dendrites. To examine the\u003cbr /\u003eco-localization of different GABA-A receptor subunits in single synapses on the soma,\u003cbr /\u003edouble labelling was performed with antibodies raised in different species. These\u003cbr /\u003eexperiments showed that the majority of synapses contains alpha1,alpha2,and beta2/3\u003cbr /\u003esubunits. Future experiments aim at testing whether distinct synapese populations\u003cbr /\u003ediffering in the relative proportions of different subunits can be detected with freeze\u003cbr /\u003efracture replica immunogold labelling.", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第963号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "20 生理科学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2005"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KASUGAI, Yu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲963_要旨.pdf", "filesize": [{"value": "168.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 168800.0, "url": {"label": "要旨・審査要旨", "url": "https://ir.soken.ac.jp/record/1165/files/甲963_要旨.pdf"}, "version_id": "7c8354d3-9558-4422-8af2-05bc4f91b1d5"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "甲963_本文.pdf", "filesize": [{"value": "18.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 18000000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/1165/files/甲963_本文.pdf"}, "version_id": "73188eb8-28c8-4fb0-b625-0f1fd2e284d0"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL)", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL)"}, {"subitem_title": "Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL)", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["22"], "permalink_uri": "https://ir.soken.ac.jp/records/1165", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1165", "relation": {}, "relation_version_is_last": true, "title": ["Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL)"], "weko_shared_id": -1}
Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL)
https://ir.soken.ac.jp/records/1165
https://ir.soken.ac.jp/records/11659b1a02af-43c1-4fe9-8be4-c6a3b9bc4539
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
||
![]() |
Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2010-02-22 | |||||
タイトル | ||||||
タイトル | Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL) | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL) | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
資源タイプ | thesis | |||||
著者名 |
春日井, 雄
× 春日井, 雄 |
|||||
フリガナ |
カスガイ, ユウ
× カスガイ, ユウ |
|||||
著者 |
KASUGAI, Yu
× KASUGAI, Yu |
|||||
学位授与機関 | ||||||
学位授与機関名 | 総合研究大学院大学 | |||||
学位名 | ||||||
学位名 | 博士(理学) | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 総研大甲第963号 | |||||
研究科 | ||||||
値 | 生命科学研究科 | |||||
専攻 | ||||||
値 | 20 生理科学専攻 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2006-03-24 | |||||
学位授与年度 | ||||||
2005 | ||||||
要旨 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Pyramidal cells in he hippocampal CA1 area express at least 14 subunits of the<br />GABA-A receptor, and receive GABAergic input from interneurons, which make<br />synapses on the soma,dendrites and the axon initial segment(AIS).Here,they used<br />the SDS-digested freeze-fracture replica labelling method to visualize the cell surface<br />distribution of the alpha1,alpha2,and beta2/3 subunits quantitatively on distinct<br />subcellular compartments of pyramidal cells. Immunogold particles for these subunits<br />were accumulated over clusters of intramembrane particles(IMP) on the protoplasmic<br />face(P-face) of the plasma membrane, indicating that many IMP clusters of a certain<br />size represent GABAergic synapses.<br />The sizes of labelled synapses were not different on the somata and the main apical<br />dendritic trunks.However,the synaptic areas on AISs were significantly smaller than<br />those on the soma and apical dendrites. Axon initial segments were identified by<br />immunogold Labelling for the Nav1.6 subunit of sodium channels. The synaptic<br />receptor labelling densities for GABA-A receptor subunits were not significantly<br />different on the tested areas of the cells. A high proportion of receptor labelling was on<br />the extrasynaptic membrane of the pyramidal cell soma and dendrites. To examine the<br />co-localization of different GABA-A receptor subunits in single synapses on the soma,<br />double labelling was performed with antibodies raised in different species. These<br />experiments showed that the majority of synapses contains alpha1,alpha2,and beta2/3<br />subunits. Future experiments aim at testing whether distinct synapese populations<br />differing in the relative proportions of different subunits can be detected with freeze<br />fracture replica immunogold labelling. | |||||
所蔵 | ||||||
値 | 有 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |