| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2010-02-22 |
| タイトル |
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タイトル |
Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL) |
| タイトル |
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タイトル |
Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL) |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
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資源タイプ |
thesis |
| 著者名 |
春日井, 雄
|
| フリガナ |
カスガイ, ユウ
|
| 著者 |
KASUGAI, Yu
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| 学位授与機関 |
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学位授与機関名 |
総合研究大学院大学 |
| 学位名 |
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学位名 |
博士（理学） |
| 学位記番号 |
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内容記述タイプ |
Other |
|
内容記述 |
総研大甲第963号 |
| 研究科 |
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|
値 |
生命科学研究科 |
| 専攻 |
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|
値 |
20 生理科学専攻 |
| 学位授与年月日 |
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学位授与年月日 |
2006-03-24 |
| 学位授与年度 |
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値 |
2005 |
| 要旨 |
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内容記述タイプ |
Other |
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内容記述 |
Pyramidal cells in he hippocampal CA1 area express at least 14 subunits of the<br />GABA-A receptor, and receive GABAergic input from interneurons, which make<br />synapses on the soma,dendrites and the axon initial segment(AIS).Here,they used<br />the SDS-digested freeze-fracture replica labelling method to visualize the cell surface<br />distribution of the alpha1,alpha2,and beta2/3 subunits quantitatively on distinct<br />subcellular compartments of pyramidal cells. Immunogold particles for these subunits<br />were accumulated over clusters of intramembrane particles(IMP) on the protoplasmic<br />face(P-face) of the plasma membrane, indicating that many IMP clusters of a certain<br />size represent GABAergic synapses.<br />The sizes of labelled synapses were not different on the somata and the main apical<br />dendritic trunks.However,the synaptic areas on AISs were significantly smaller than<br />those on the soma and apical dendrites. Axon initial segments were identified by<br />immunogold Labelling for the Nav1.6 subunit of sodium channels. The synaptic<br />receptor labelling densities for GABA-A receptor subunits were not significantly<br />different on the tested areas of the cells. A high proportion of receptor labelling was on<br />the extrasynaptic membrane of the pyramidal cell soma and dendrites. To examine the<br />co-localization of different GABA-A receptor subunits in single synapses on the soma,<br />double labelling was performed with antibodies raised in different species. These<br />experiments showed that the majority of synapses contains alpha1,alpha2,and beta2/3<br />subunits. Future experiments aim at testing whether distinct synapese populations<br />differing in the relative proportions of different subunits can be detected with freeze<br />fracture replica immunogold labelling. |
| 所蔵 |
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値 |
有 |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
要旨・審査要旨 (168.8 kB)
