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Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL)
https://ir.soken.ac.jp/records/1165
https://ir.soken.ac.jp/records/11659b1a02af-43c1-4fe9-8be4-c6a3b9bc4539
名前 / ファイル | ライセンス | アクション |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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公開日 | 2010-02-22 | |||||||
タイトル | ||||||||
タイトル | Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL) | |||||||
タイトル | ||||||||
タイトル | Quantitative localization of GABAA receptor subunits on hippocampal pyramidal cells by SDS-digested freeze fracture replica labeling(SDS-FRL) | |||||||
言語 | en | |||||||
言語 | ||||||||
言語 | eng | |||||||
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資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||||
資源タイプ | thesis | |||||||
著者名 |
春日井, 雄
× 春日井, 雄
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フリガナ |
カスガイ, ユウ
× カスガイ, ユウ
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著者 |
KASUGAI, Yu
× KASUGAI, Yu
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学位授与機関 | ||||||||
学位授与機関名 | 総合研究大学院大学 | |||||||
学位名 | ||||||||
学位名 | 博士(理学) | |||||||
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内容記述タイプ | Other | |||||||
内容記述 | 総研大甲第963号 | |||||||
研究科 | ||||||||
値 | 生命科学研究科 | |||||||
専攻 | ||||||||
値 | 20 生理科学専攻 | |||||||
学位授与年月日 | ||||||||
学位授与年月日 | 2006-03-24 | |||||||
学位授与年度 | ||||||||
値 | 2005 | |||||||
要旨 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | Pyramidal cells in he hippocampal CA1 area express at least 14 subunits of the<br />GABA-A receptor, and receive GABAergic input from interneurons, which make<br />synapses on the soma,dendrites and the axon initial segment(AIS).Here,they used<br />the SDS-digested freeze-fracture replica labelling method to visualize the cell surface<br />distribution of the alpha1,alpha2,and beta2/3 subunits quantitatively on distinct<br />subcellular compartments of pyramidal cells. Immunogold particles for these subunits<br />were accumulated over clusters of intramembrane particles(IMP) on the protoplasmic<br />face(P-face) of the plasma membrane, indicating that many IMP clusters of a certain<br />size represent GABAergic synapses.<br />The sizes of labelled synapses were not different on the somata and the main apical<br />dendritic trunks.However,the synaptic areas on AISs were significantly smaller than<br />those on the soma and apical dendrites. Axon initial segments were identified by<br />immunogold Labelling for the Nav1.6 subunit of sodium channels. The synaptic<br />receptor labelling densities for GABA-A receptor subunits were not significantly<br />different on the tested areas of the cells. A high proportion of receptor labelling was on<br />the extrasynaptic membrane of the pyramidal cell soma and dendrites. To examine the<br />co-localization of different GABA-A receptor subunits in single synapses on the soma,<br />double labelling was performed with antibodies raised in different species. These<br />experiments showed that the majority of synapses contains alpha1,alpha2,and beta2/3<br />subunits. Future experiments aim at testing whether distinct synapese populations<br />differing in the relative proportions of different subunits can be detected with freeze<br />fracture replica immunogold labelling. | |||||||
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値 | 有 | |||||||
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内容記述タイプ | Other | |||||||
内容記述 | application/pdf |