ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

  1. 020 学位論文
  2. 生命科学研究科
  3. X2 分子生物機構論専攻

Restriction mechanism of bone morphogenetic protein in the early development of Xenopus Laevis

https://ir.soken.ac.jp/records/1362
https://ir.soken.ac.jp/records/1362
5e8aae79-1267-4866-acf1-6977908b8168
名前 / ファイル ライセンス アクション
甲610_要旨.pdf 要旨・審査要旨 / Abstract, Screening Result (192.9 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2010-02-22
タイトル
タイトル Restriction mechanism of bone morphogenetic protein in the early development of Xenopus Laevis
タイトル
タイトル Restriction mechanism of bone morphogenetic protein in the early development of Xenopus Laevis
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_46ec
資源タイプ thesis
著者名 大河原, 美静

× 大河原, 美静

大河原, 美静

Search repository
フリガナ オオカワラ, ビセイ

× オオカワラ, ビセイ

オオカワラ, ビセイ

Search repository
著者 OHKAWARA, Bisei

× OHKAWARA, Bisei

en OHKAWARA, Bisei

Search repository
学位授与機関
学位授与機関名 総合研究大学院大学
学位名
学位名 博士(理学)
学位記番号
内容記述タイプ Other
内容記述 総研大甲第610号
研究科
値 生命科学研究科
専攻
値 X2 分子生物機構論専攻
学位授与年月日
学位授与年月日 2002-03-22
学位授与年度
値 2001
要旨
内容記述タイプ Other
内容記述 During early development, cells receive positional information from neighboring cells that direct how they should differentiate to form tissue patterns from initially uniform germ layers.Ligands of the transforming growth factor (TGF-β) superfamily are known to participate in this pattern formation. In particular, activin has been shown to act as a long-range signal to establish a concentration gradient in X enopus ectoderm.In contrast, the action of BMP-2 and BMP-4, both of which also belong to this superfamily, appears to be restricted to the ventral ectoderm where they induce the epidermal fate in a tightly localized manner.This raises a question as to how the action of BMP is tightly restricted to within and around the cells that produce it.<br />To investigate this question, I noticed that some of the BMP subfamily ligands contain a unique core of basic amino acids in their N-terminus.Then cDNA encoding BMP-4 variants, lacking basic amino acids core in their N-terminal region, were constructed.To analyze their action ranges in Xenopus embryo, the experiments using presumptive ectoderm and whole embryo were preformed.Results are, first, that a basic core of only three amino acids in the N-terminal region of BMP-4 is required not for its biological activity but for its restriction to the non-neural ectoderm and to the ventral tissue. Second, I demonstrated that heparan sulfate proteoglycans (HSPGs) bind to this basic core and WTBMP-4 could act as long-range siganl molecules where HSPGs are absent in vivo.<br />These observations suggest that the basic amino acids in the N-terminal region of BMP-4 and particularly the basic core play an essential role in conferring to BMP-4 a short-range action in vivo.And I demonstrated that at least one of the molecules trapping WTBMP-4 on the surface of animal cap cells is HSPGs in vivo.The present work first identifies the critical domain of BMP for the restriction of its diffusion through the interaction with extracellular environments in vivo.
所蔵
値 有
戻る
0
views
See details
Views

Versions

Ver.1 2023-06-20 14:44:48.538528
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR 2.0
  • OAI-PMH JPCOAR 1.0
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3