| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-11-06 |
| タイトル |
|
|
タイトル |
Room-Temperature Solution Fluorescence Excitation Correlation Spectroscopy |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
single-molecule spectroscopy |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
fluorescence correlation spectroscopy |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
excitation spectrum |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Yusuke Yoneda
Hikaru Kuramochi
|
| 著者別名 |
米田, 勇祐
倉持, 光
|
| 著者注記 |
|
|
言語 |
en |
|
値 |
Research Center of Integrative Molecular Systems (CIMoS), Institute for Molecular Science, National Institutes of Natural Sciences, 38 Nishigo-Naka, Myodaiji, Okazaki 444-8585, Japan |
| 著者注記 |
|
|
言語 |
en |
|
値 |
Graduate Institute for Advanced Studies, SOKENDAI, 38 Nishigo-Naka, Myodaiji, Okazaki 444-8585, Japan |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Single-molecule fluorescence spectroscopy is a powerful tool to investigate the physical properties of individual molecules. Yet, elucidating the fast fluctuation dynamics of freely diffusing single molecules in solution at room temperature, where a variety of chemical and biological processes occur, remains challenging. In this study, we report on fluorescence excitation correlation spectroscopy (FECS) of room-temperature solutions, which enables the study of spontaneous fluctuation of the excitation spectrum with microsecond time resolution. By employing Fourier transform spectroscopy with broadband femtosecond pulses and time-correlated single-photon counting, we achieved fluorescence excitation spectroscopy of a roomtemperature
solution at the single-molecule level. Building upon this single-molecule measurement, we obtained an excitation wavelength-resolved fluorescence autocorrelation function in the microsecond to millisecond range, demonstrating the potential of this method to elucidate fast, spontaneous, timedependent changes of excitation spectra in statistically equilibrium systems. With further development, this method will allow the study of spectral exchange associated with transitions between sub-ensembles of solution-phase molecules with unprecedented time resolution. |
|
言語 |
en |
| 論文注記 |
|
|
言語 |
en |
|
値 |
This document is the Accepted Manuscript version of a Published Work that appeared in final form in [The Journal of Physical Chemistry Letters], copyright © [2024 American Chemical Society] after peer review and technical editing by the publisher. To access the final edited and published work see [https://doi.org/10.1021/acs.jpclett.4c01798]. |
| 書誌情報 |
en : The Journal of Physical Chemistry Letters
巻 15,
号 33,
p. 8533-8539,
ページ数 7,
発行日 2024-08-12
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| 出版者 |
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|
出版者 |
American Chemical Society |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1948-7185 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1021/acs.jpclett.4c01798 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
© 2024 American Chemical Society |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
本文 (5.9 MB)
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