| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2012-01-10 |
| タイトル |
|
|
タイトル |
Localized and Ran-dependent accumulation of tubulin during semi-open mitosis in the Caenorhabditis elegans embryo |
| タイトル |
|
|
タイトル |
Localized and Ran-dependent accumulation of tubulin during semi-open mitosis in the Caenorhabditis elegans embryo |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 著者名 |
林, 華子
|
| フリガナ |
ハヤシ , ハナコ
|
| 著者 |
HAYASHI, Hanako
|
| 学位授与機関 |
|
|
|
学位授与機関名 |
総合研究大学院大学 |
| 学位名 |
|
|
学位名 |
博士（理学） |
| 学位記番号 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
総研大甲第1434号 |
| 研究科 |
|
|
値 |
生命科学研究科 |
| 専攻 |
|
|
値 |
18 遺伝学専攻 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2011-03-24 |
| 学位授与年度 |
|
|
値 |
2010 |
| 要旨 |
|
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内容記述タイプ |
Other |
|
内容記述 |
Mitosis is categorized as open or closed depending on whether loss of nuclear compartmentalization occurs or not. Some organisms adopt intermediate forms of mitosis, which are important to understand the commonality and diversity of the process. Caenorhabditis elegans undergoes semi-open mitosis. In this process, the permeability of the nuclear envelope increases during the prometaphase, but the membrane structure remains and encloses the mitotic spindle. The role of the remaining permeabilized nuclear envelope (in this thesis, I refer to the envelope as “spindle envelope”) is still unclear. Here, I report that unpolymerized tubulin subunits accumulate inside the spindle envelope in the C. elegans embryo. The accumulation coincided with increased nuclear envelope permeability, suggesting that the rise in permeability triggers the accumulation. A small GTPase protein, Ran, which plays a key role in providing spatial cues during open and closed mitosis, is required for tubulin accumulation. The boundaries of the region of accumulation coincided with the spindle envelope even when the shape of the envelope was deformed. Fluorescence recovery after photobleaching (FRAP) analysis revealed the enrichment of the immobile fraction in the nascent spindle region in a Ran-dependent manner. I propose stable binding of tubulin to an immobile structure as a mechanism of tubulin accumulation inside the spindle envelope. The accumulation was also observed for molecules other than tubulin, suggesting that this novel mechanism contributes to alter the molecular composition around the chromosome during semi-open mitosis in the C. elegans embryo. |
| 所蔵 |
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|
値 |
有 |
要旨・審査要旨 (404.9 kB)
