| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2012-12-11 |
| タイトル |
|
|
タイトル |
Immunohistochemical localization of Papilio RBP in the eye of butterflies |
| タイトル |
|
|
タイトル |
Immunohistochemical localization of Papilio RBP in the eye of butterflies |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
butterfly,Papilio xuthus, Papilionoidea,retinoid binding protein,immunohistochemistry,3-hydroxyretinol,pigment cell,tracheal cell,visual cycle |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
WAKAKUWA, Motohiro
OZAKI, Koichi
ARIKAWA, Kentaro
|
| 著者別名 |
若桑 , 基博
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We recently identified a novel retinoid binding protein, Papilio RBP, in the soluble fraction of the eye homogenate of the butterfly Papilio xuthus, and demonstrated that the protein is involved in the visual cycle. We now have localized the protein in the Papilio eye by light and electron microscopic immunohistochemistry using a monospecific antiserum produced against artificially expressed Papilio RBP. We found strong immunoreactivity in the primary as well as secondary pigment cells and in the tracheal cells. The pigment cells have long been regarded as an important site of the visual cycle, and this view is further supported by the present result. Interestingly, the cytoplasm and nuclei of these cells were equally labeled, indicating that the protein exists in both the cytoplasm and the nucleus. We conducted a survey for the existence of the Papilio RBP-like proteins in other insects including several species of butterflies, dragonflies, cicadas, grasshoppers and honeybees. Anti-Papilio RBP immunoreactivity was confirmed in the proteins isolated only from butterflies belonging to the superfamily Papilionoidea and not from other species. In all insects tested, however, fluorescing proteins were clearly detected, suggesting that these insects also have similar retinol-binding proteins. |
| 書誌情報 |
Journal of Experimental Biology
en : Journal of Experimental Biology
巻 207,
号 9,
p. 1479-1486,
発行日 2004-02-02
|
| 出版者 |
|
|
出版者 |
The Company of Biologists Ltd |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00220949 |
| PubMed番号 |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
15037642 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://doi.org/10.1242/jeb.00913 |
|
|
関連名称 |
10.1242/jeb.00913 |
| 権利 |
|
|
権利情報 |
© The Company of Biologists Limited 2004 |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://www.biologists.com/dmm/dmm_information.html#anchor_access |
|
|
関連名称 |
Copyright |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
1479.full (452.5 kB)
