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  1. 010 学術雑誌論文
  2. 蟻川, 謙太郎 / ARIKAWA, Kentaro
  1. 010 学術雑誌論文
  2. 01 研究員発表論文
  3. 若桑, 基博 / WAKAKUWA, Motohiro

Immunohistochemical localization of Papilio RBP in the eye of butterflies

https://ir.soken.ac.jp/records/3265
https://ir.soken.ac.jp/records/3265
f211e16b-751f-4c25-9aa7-7168876b90f4
名前 / ファイル ライセンス アクション
1479.full.pdf 1479.full (452.5 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2012-12-11
タイトル
タイトル Immunohistochemical localization of Papilio RBP in the eye of butterflies
タイトル
タイトル Immunohistochemical localization of Papilio RBP in the eye of butterflies
言語 en
言語
言語 eng
キーワード
言語 en
主題Scheme Other
主題 butterfly,Papilio xuthus, Papilionoidea,retinoid binding protein,immunohistochemistry,3-hydroxyretinol,pigment cell,tracheal cell,visual cycle
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
著者 WAKAKUWA, Motohiro

× WAKAKUWA, Motohiro

WAKAKUWA, Motohiro

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OZAKI, Koichi

× OZAKI, Koichi

OZAKI, Koichi

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ARIKAWA, Kentaro

× ARIKAWA, Kentaro

ARIKAWA, Kentaro

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著者別名 若桑 , 基博

× 若桑 , 基博

若桑 , 基博

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抄録
内容記述タイプ Abstract
内容記述 We recently identified a novel retinoid binding protein, Papilio RBP, in the soluble fraction of the eye homogenate of the butterfly Papilio xuthus, and demonstrated that the protein is involved in the visual cycle. We now have localized the protein in the Papilio eye by light and electron microscopic immunohistochemistry using a monospecific antiserum produced against artificially expressed Papilio RBP. We found strong immunoreactivity in the primary as well as secondary pigment cells and in the tracheal cells. The pigment cells have long been regarded as an important site of the visual cycle, and this view is further supported by the present result. Interestingly, the cytoplasm and nuclei of these cells were equally labeled, indicating that the protein exists in both the cytoplasm and the nucleus. We conducted a survey for the existence of the Papilio RBP-like proteins in other insects including several species of butterflies, dragonflies, cicadas, grasshoppers and honeybees. Anti-Papilio RBP immunoreactivity was confirmed in the proteins isolated only from butterflies belonging to the superfamily Papilionoidea and not from other species. In all insects tested, however, fluorescing proteins were clearly detected, suggesting that these insects also have similar retinol-binding proteins.
書誌情報 Journal of Experimental Biology
en : Journal of Experimental Biology

巻 207, 号 9, p. 1479-1486, 発行日 2004-02-02
出版者
出版者 The Company of Biologists Ltd
ISSN
収録物識別子タイプ ISSN
収録物識別子 00220949
PubMed番号
関連タイプ isIdenticalTo
識別子タイプ PMID
関連識別子 15037642
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 http://doi.org/10.1242/jeb.00913
関連名称 10.1242/jeb.00913
権利
権利情報 © The Company of Biologists Limited 2004
関連サイト
識別子タイプ URI
関連識別子 http://www.biologists.com/dmm/dmm_information.html#anchor_access
関連名称 Copyright
フォーマット
内容記述タイプ Other
内容記述 application/pdf
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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