| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-01-21 |
| タイトル |
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タイトル |
Photomanipulation of antibiotic susceptibility and biofilm formation of Escherichia coli heterologously expressing photoactivated adenylyl cyclase |
| タイトル |
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タイトル |
Photomanipulation of antibiotic susceptibility and biofilm formation of Escherichia coli heterologously expressing photoactivated adenylyl cyclase |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
Antibiotics,Biofilm,cAMP,Escherichia coil,Photomanipulation |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
YASUKAWA, Hiro
KONNO, Noriko
HANEDA, Yukari
YAMAMORI, Baku
ISEKI, Mineo
SHIBUSAWA, Mami
ONO, Yasushi
KODAIRA, Ken-ichi
FUNADA, Hisashi
WATANABE, Masakatsu
|
| 著者別名 |
渡辺, 正勝
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
A cyaA-deficient Escherichia coli strain was transformed by a plasmid carrying the gene for BsPAC, a photoactivated adenylyl cyclase identified from a Beggiatoa sp., and was subjected to an antibiotic susceptibility assay and biofilm formation assay under a light or dark condition. Cells expressing BsPAC that were incubated under blue light (470 nm) were more susceptible to fosfomycin, nalidixic acid and streptomycin than were cells incubated in the dark. Cells expressing BsPAC formed more biofilms when incubated under the light than did cells cultured in the dark. We concluded from these observations that it is possible to determine the importance of cAMP in antibiotic susceptibility and biofilm formation of E. coli by photomanipulating the cellular cAMP level by the use of BsPAC. A site-directed mutant of BsPAC in which Tyr7 was replaced by Phe functioned even in the dark, indicating that Tyr7 plays an important role in photoactivation of BsPAC. Results of mutational analysis of BsPAC should contribute to an understanding of the molecular basis for photoactivation of the protein. |
| 書誌情報 |
Journal of General and Applied Microbiology
en : Journal of General and Applied Microbiology
巻 58,
号 3,
p. 183-190,
発行日 2012-08-08
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| 出版者 |
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出版者 |
The Microbiology Research Foundation |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
00221260 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.2323/jgam.58.183 |
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関連名称 |
10.2323/jgam.58.183 |
| 権利 |
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権利情報 |
© 2012, The Microbiology Research Foundation |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
https://www.jstage.jst.go.jp/article/jgam/58/3/58_183/_article |
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関連名称 |
J-STAGE |
| 関連サイト |
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識別子タイプ |
URI |
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|
関連識別子 |
https://www.jstage.jst.go.jp/pub/html/AY04S110_ja.html |
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関連名称 |
Copyright |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
58_183_pdf (15.1 MB)
