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Distinct responses of chloroplasts to blue and green laser microbeam irradiations in the centric diatom Pleurosira laevis
https://ir.soken.ac.jp/records/3330
https://ir.soken.ac.jp/records/33309f744e0f-8f6d-4395-8585-3b6a5610349c
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-01-21 | |||||
| タイトル | ||||||
| タイトル | Distinct responses of chloroplasts to blue and green laser microbeam irradiations in the centric diatom Pleurosira laevis | |||||
| タイトル | ||||||
| タイトル | Distinct responses of chloroplasts to blue and green laser microbeam irradiations in the centric diatom Pleurosira laevis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
SHIHIRA-ISHIKAWA, Ikuko
× SHIHIRA-ISHIKAWA, Ikuko× NAKAMURA, Takanori× HIGASHI, Sho-ichi× WATANABE, Masakatsu |
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| 著者別名 |
渡辺, 正勝
× 渡辺, 正勝 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The centric diatomPleurosira laevis is a large unicellular alga, in which ca 200 chloroplasts migrate toward the nuclear cytoplasm through the transvacuolar cytoplasmic strands in response to blue-light irradiation and, on the contrary, toward the cortical cytoplasm in response to green-light irradiation. We analyzed these light-induced chloroplast migrations using a scanning laser microbeam provided by a confocal microscope for intracellular irradiation. Spot irradiation of a blue laser microbeam induced rapid assemblage of chroloplasts into the nuclear cytoplasm regardless of the spot position and spot number. On the other hand, one or two spots of green laser microbeam induced chloroplast accumulation at the spots, although increasing spot numbers suppressed chloroplast accumulation at each spot. In our experimental condition, ca 1 min of blue-light irradiation was sufficient to stimulate movement, whereas green-light irradiation required uninterrupted and longer irradiation time (ca 15 min). Chloroplast assemblage induced by blue-light required extracellular Ca 2+, and was inhibited by Ca 2+channel antagonists. Furthermore, higher efficiencies of chloroplast migration were obtained when a single beam spot was fragmented and scattered over wider area of plasma membrane. These observations suggested that blue-light induced a response at the plasma membrane, which subsequently activated Ca 2+permeable channels. This sequence of physiological events is identical to what was previously observed with chloroplast movement in response to mechanical stimulation. Furthermore, experiments with the cytoskeleton-disrupting agents, colchicine and cytochalasin D, indicated that blue-light-induced chloroplast movement required microtubules whereas the green-light-induced response to beam spot required actin filaments. | |||||
| 書誌情報 |
Photochemical and Photobiological Sciences en : Photochemical and Photobiological Sciences 巻 83, 号 5, p. 1101-1109, 発行日 2007-05-09 |
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| 出版者 | ||||||
| 出版者 | Royal Society of Chemistry | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00318655 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 17880505 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/j.1751-1097.2007.00167.x | |||||
| 関連名称 | 10.1111/j.1751-1097.2007.00167.x | |||||
| 権利 | ||||||
| 権利情報 | © 1999–2013 John Wiley & Sons, Inc | |||||
