| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-05 |
| タイトル |
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|
タイトル |
Validation of salivary cortisol and testosterone assays in chimpanzees by liquid chromatography-tandem mass spectrometry |
| タイトル |
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タイトル |
Validation of salivary cortisol and testosterone assays in chimpanzees by liquid chromatography-tandem mass spectrometry |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
KUTSUKAKE, Nobuyuki
IKEDA, Koki
HONMA, Seijiro
TERAMOTO, Migaku
MORI, Yusuke
HAYASAKA, Ikuo
YAMAMOTO, Rain
ISHIDA, Takafumi
YOSHIKAWA, Yasuhiro
HASEGAWA, Toshikazu
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| 著者別名 |
沓掛, 展之
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Owing to its high temporal sensitivity, saliva has distinct advantages for measuring steroids, compared with other noninvasive samples such as urine and feces. Here, we report the validity of assaying salivary cortisol (C) and testosterone (T) using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in captive male chimpanzees, Pan troglodytes. For both the C and T concentrations, we found positive relationships between saliva and plasma. The concentrations of C and T in saliva showed clear patterns of diurnal fluctuation, whereas those in urine and feces did not. These results suggest that the salivary steroid concentrations can be regarded as good indicators of circulating steroid levels. We also developed and validated an efficient method for collecting saliva samples from cotton rope. Although rope includes inherent steroid-like compounds and may affect the accuracy of steroid measurements, our rope-washing procedures effectively removed intrinsic steroidal materials. There was a significant association between the C and T concentrations measured from saliva collected from rope licked by the chimpanzees and those measured from saliva collected directly from the mouth. Salivary T values estimated by LC/MS-MS were similar to those measured by radioimmunoassay. The results indicate the usefulness of saliva as a noninvasive steroid measure and that steroids in the saliva of chimpanzees can be accurately measured by LC-MS/MS. Am. J. Primatol. 71:696–706, 2009. |
| 書誌情報 |
American Journal of Primatology
en : American Journal of Primatology
巻 71,
号 8,
p. 696-706,
発行日 2009-08
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| 出版者 |
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出版者 |
Wiley-Blackwell |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
02752565 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
19452511 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1002/ajp.20708 |
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関連名称 |
10.1002/ajp.20708 |
| 権利 |
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権利情報 |
© 2009 Wiley-Liss, Inc |
