| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-09 |
| タイトル |
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タイトル |
Very strong UV-A light temporally separates the photoinhibition of photosystem II into light-induced inactivation and repair |
| タイトル |
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タイトル |
Very strong UV-A light temporally separates the photoinhibition of photosystem II into light-induced inactivation and repair |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
ZSIROS, Otto
ALLAKHVERDIEV, Suleyman I
HIGASHI, Shoichi
WATANABE, Masakatsu
NISHIYAMA, Yoshitaka
MURATA, Norio
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| 著者別名 |
渡辺, 正勝
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
When organisms that perform oxygenic photosynthesis are exposed to strong visible or UV light, inactivation of photosystem II (PSII) occurs. However, such organisms are able rapidly to repair the photoinactivated PSII. The phenomenon of photoinactivation and repair is known as photoinhibition. Under normal laboratory conditions, the rate of repair is similar to or faster than the rate of photoinactivation, preventing the detailed analysis of photoinactivation and repair as separate processes. We report here that, using strong UV-A light from a laser, we were able to analyze separately the photoinactivation and repair of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. Very strong UV-A light at 364 nm and a photon flux density of 2600 μmol photons m -2 s-1 inactivated the oxygen-evolving machinery and the photochemical reaction center of PSII within 1 or 2 min before the first step in the repair process, namely, the degradation of the D1 protein, occurred. During subsequent incubation of cells in weak visible light, the activity of PSII recovered fully within 30 min and this process depended on protein synthesis. During subsequent incubation of cells in darkness for 60 min, the D1 protein of the photoinactivated PSII was degraded. Further incubation in weak visible light resulted in the rapid restoration of the activity of PSII. These observations suggest that very strong UV-A light is a useful tool for the analysis of the repair of PSII after photoinactivation. |
| 書誌情報 |
Biochimica et Biophysica Acta - Bioenergetics
en : Biochimica et Biophysica Acta - Bioenergetics
巻 1757,
号 2,
p. 123-129,
発行日 2006-02
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
00052728 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
16500615 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.bbabio.2006.01.004 |
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関連名称 |
10.1016/j.bbabio.2006.01.004 |
| 権利 |
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権利情報 |
© 2006 Elsevier B.V. |
