| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-09 |
| タイトル |
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タイトル |
Characterization of arylalkylamine N-acetyltransferase (AANAT) activities and action spectrum for suppression in the band-legged cricket, Dianemobius nigrofasciatus (Orthoptera: Gryllidae) |
| タイトル |
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タイトル |
Characterization of arylalkylamine N-acetyltransferase (AANAT) activities and action spectrum for suppression in the band-legged cricket, Dianemobius nigrofasciatus (Orthoptera: Gryllidae) |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
IZAWA, Norimitsu
SUZUKI, Takeshi
WATANABE, Masakatsu
TAKEDA, Makio
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| 著者別名 |
渡辺, 正勝
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Arylalkylamine N-acetyltransferase (AANAT), constituting a large family of enzymes, catalyzes the transacetylation from acetyl-CoA to monoamine substrates, although homology among species is not very high. AANAT in vertebrates is photosensitive and mediates circadian regulation. Here, we analyzed AANAT of the cricket, Dianemobius nigrofasciatus. The central nervous system contained AANAT activity. The optimum pHs were 6.0 (a minor peak) and 10.5 (a major peak) with crude enzyme solution. We analyzed the kinetics at pH 10.5 using the sample containing collective AANAT activities, which we term AANAT. Lineweaver-Burk plot and secondary plot yielded a Km for tryptamine as substrate of 0.42 μM, and a Vmax of 9.39 nmol/mg protein/min. The apparent Km for acetyl-CoA was 59.9 μM and the Vmax was 8.14 nmol/mg protein/min. AANAT of D. nigrofasciatus was light-sensitive. The activity was higher at night-time than at day-time as in vertebrates. To investigate most effective wavelengths on AANAT activity, a series of monochromatic lights was applied (350, 400, 450, 500, 550, 600 and 650 nm). AANAT showed the highest sensitivity to around 450 nm and 550 nm. 450 nm light was more effective than 550 nm light. Therefore, the most effective light affecting AANAT activity is blue light, which corresponds to the absorption spectrum of blue wave (BW)-opsin. |
| 書誌情報 |
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
en : Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
巻 152,
号 4,
p. 346-351,
発行日 2009-04
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
10964959 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
19168144 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.cbpb.2008.12.016 |
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関連名称 |
10.1016/j.cbpb.2008.12.016 |
| 権利 |
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権利情報 |
© 2009 Published by Elsevier Inc. |
