| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-09 |
| タイトル |
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タイトル |
Somatic cell mutation and photoproduct formation in Drosophila induced by monochromatic UV light in sunlight |
| タイトル |
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タイトル |
Somatic cell mutation and photoproduct formation in Drosophila induced by monochromatic UV light in sunlight |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
TAKINAMI, Shogo
MOCHIZUKI, Misato
HAYATSU, Hikoya
NIKAIDO, Osamu
KUBOTA, Mamoru
WATANABE, Masakatsu
HIEDA, Miwako
HIEDA, Kotaro
NEGISHI, Tomoe
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| 著者別名 |
渡辺, 正勝
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We recently reported that natural sunlight can induce somatic mutation and chromosomal recombination in Drosophila, as detected by the wing spot test. Simultaneously, cyclobutane thymine dimers (CTDs) and (6-4)photoproducts [(6-4)PPs] are formed in the DNA. In the present work, we have performed monochromatic UV irradiation for evaluating the action spectrum of sunlight in genotoxicity. Third instar larvae in petri dishes were irradiated by monochromatic UV light at a wavelength between 310 and 360 nm. The mutant spots induced by the irradiation of 310, 320, 330, and 340 nm light were (4.4 ± 1.0) x 10 -1, (1.1 ± 0.2) x 10 -2, (2.5 ± 0.7) x 10 -3, and (2.0 ± 0.7) x 10 -3 spots/wing/kJ/m 2, respectively. The mutagenicity at 360 nm irradiation was not significant. The amounts of photoproducts in irradiated larval DNA were measured by an enzyme-linked immunosorbent assay (ELISA) using the monoclonal antibodies against CTDs and (6-4)PPs. CTDs were found in the DNA from all of these irradiated larvae, and their amounts increased with the UV dose: the longer the wavelength, the lower the CTD formation and the mutagenesis. These data suggest that, in the sunlight, the component around 310-340 nm causes the somatic cell mutation by forming thymine dimers as main lesions. |
| 書誌情報 |
Environmental Toxicology
en : Environmental Toxicology
巻 15,
号 5,
p. 496-499,
発行日 2000
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| 出版者 |
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出版者 |
Wiley-Blackwell |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
15204081 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1002/1522-7278(2000)15:5<496::AID-TOX18>3.0.CO;2-M |
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関連名称 |
10.1002/1522-7278(2000)15:5<496::AID-TOX18>3.0.CO;2-M |
| 権利 |
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権利情報 |
© 2000 by John Wiley and Sons, Inc |
