| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-12 |
| タイトル |
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タイトル |
UVA1 genotoxicity is mediated not by oxidative damage but by cyclobutane pyrimidine dimers in normal mouse skin |
| タイトル |
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タイトル |
UVA1 genotoxicity is mediated not by oxidative damage but by cyclobutane pyrimidine dimers in normal mouse skin |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
IKEHATA, Hironobu
KAWAI, Kazuaki
KOMURA, Jun-ichiro
SAKATSUME, Ko
WANG, Liangcheng
IMAI, Masaru
HIGASHI, Shoichi
NIKAIDO, Osamu
YAMAMOTO, Kazuo
HIEDA, Kotaro
WATANABE, Masakatsu
KASAI, Hiroshi
ONO, Tetsuya
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| 著者別名 |
渡辺, 正勝
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
UVA1 induces the formation of 8-hydroxy-2′-deoxyguanosines (8-OH-dGs) and cyclobutane pyrimidine dimers (CPDs) in the cellular genome. However, the relative contribution of each type of damage to the in vivo genotoxicity of UVA1 has not been clarified. We irradiated living mouse skin with 364-nm UVA1 laser light and analyzed the DNA damage formation and mutation induction in the epidermis and dermis. Although dose-dependent increases were observed for both 8-OH-dG and CPD, the mutation induction in the skin was found to result specifically from the CPD formation, based on the induced mutation spectra in the skin genome: the dominance of C → T transition at a dipyrimidine site. Moreover, these UV-specific mutations occurred preferentially at the 5′-TCG-3′ sequence, suggesting that CpG methylation and photosensitization-mediated triplet energy transfer to thymine contribute to the CPD-mediated UVA1 genotoxicity. Thus, it is the CPD formation, not the oxidative stress, that effectively brings about the genotoxicity in normal skin after UVA1 exposure. We also found differences in the responses to the UVA1 genotoxicity between the epidermis and the dermis: the mutation induction after UVA1 irradiation was suppressed in the dermis at all levels of irradiance examined, whereas it leveled off from a certain high irradiance in the epidermis. |
| 書誌情報 |
Journal of Investigative Dermatology
en : Journal of Investigative Dermatology
巻 128,
号 9,
p. 2289-2296,
発行日 2008-09
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| 出版者 |
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出版者 |
Nature Publishing Group |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0022202X |
| PubMed番号 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
PMID |
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関連識別子 |
18356809 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1038/jid.2008.61 |
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関連名称 |
10.1038/jid.2008.61 |
| 権利 |
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権利情報 |
© 2008 The Society for Investigative Dermatology |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
http://www.nature.com/authors/policies/confidentiality.html |
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関連名称 |
Copyright |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
jid200861a (216.0 kB)
