| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-12 |
| タイトル |
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|
タイトル |
Vibrational microspectroscopy of single proteins |
| タイトル |
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|
タイトル |
Vibrational microspectroscopy of single proteins |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
FUJIYOSHI, Satoru
FURUYA, Yo
ISEKI, Mineo
WATANABE, Masakatsu
MATSUSHITA, Michio
|
| 著者別名 |
渡辺, 正勝
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Vibrational infrared absorption of a single protein molecule was detected at a few kelvins as infrared-induced recovery of visible fluorescence of a dye with which the protein was labeled. This sensitive method of detecting infrared absorption was demonstrated for a single bovine serum albumin (BSA) molecule labeled with Alexa Fluor 660 by determining the vibrational infrared absorption spectrum of the backbone vibrations of the α-helical structure in the wavelength region around 6 μm (1650 cm -1). In addition to measuring the vibrational infrared absorption spectrum, the visible fluorescence can be simultaneously used for imaging of the same dye-labeled single protein molecules. |
| 書誌情報 |
Journal of Physical Chemistry Letters
en : Journal of Physical Chemistry Letters
巻 1,
号 17,
p. 2541-2545,
発行日 2010-09-02
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| 出版者 |
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出版者 |
ACP Publications |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
19487185 |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
http://doi.org/10.1021/jz100858b |
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関連名称 |
10.1021/jz100858b |
| 権利 |
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権利情報 |
© 2010 American Chemical Society |
