| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-13 |
| タイトル |
|
|
タイトル |
Time course and spacial distribution of UV effects on human skin in organ culture |
| タイトル |
|
|
タイトル |
Time course and spacial distribution of UV effects on human skin in organ culture |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
metadata only access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
MORI, Eiichiro
TAKAHASHI, Akihisa
KITAGAWA, Kou
KAKEI, Saki
TSUJINAKA, Daiki
UNNO, Megumi
NISHIKAWA, Shoko
OHNISHI, Ken
HATOKO, Mitsuo
MURATA, Norio
WATANABE, Masakatsu
FURUSAWA, Yoshiya
OHNISHI, Takeo
|
| 著者別名 |
渡辺, 正勝
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Apoptosis plays an important role in eliminating cells from populations when cells have been exposed to UV irradiation and damaged. Studies of cells in culture have provided some details of the mechanisms involved when stress response genes act after exposure to UV irradiation and other environmental stresses. However, little is known about the responses of intact sections of human skin growing in organ culture to UV irradiation. In the work reported here, it was found that the response of organ-cultured human skin after exposure to UV irradiation is different than the response of cultured cells. At wavelengths below 300 nm, the action spectrum obtained from organ-cultured skin samples showed a lower sensitivity than that observed at 300 nm, indicating that the overlying stratum corneum and upper epidermal cell layers had probably caused a selective absorption of incident UV radiation at some wavelengths. At 3 hours after UV irradiation, p53 was phosphorylated at Ser 15 and Ser 46, and accumulated in the cell nuclei, notably after exposure to 280-320 nm wavelengths. Accumulations of Bax, active Caspase-3 and cleaved PARP were detected in apoptotic cells at 24 hours post-exposure, along with a reduction of Bcl-2 levels, notably after exposure to 300-365 nm light. This difference in apoptotic responses may result from the characteristics of the different irradiation wavelengths used, and from details in the skin's structure. The data obtained in this study using an organ-culture system utilized direct measurements of the biological effects of different wavelengths of UV lights. |
| 書誌情報 |
Journal of Radiation Research
en : Journal of Radiation Research
巻 49,
号 3,
p. 269-277,
発行日 2008
|
| 出版者 |
|
|
出版者 |
Oxford University Press |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
04493060 |
| PubMed番号 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
18311036 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1269/jrr.07106 |
|
|
関連名称 |
10.1269/jrr.07106 |
| 権利 |
|
|
権利情報 |
© 2008 Japan Radiation Research Society |
