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Kinetic analysis of the activation of photoactivated adenylyl cyclase (PAC), a blue-light receptor for photomovements of Euglena
https://ir.soken.ac.jp/records/3886
https://ir.soken.ac.jp/records/3886864ce08d-415b-463c-95c0-ae04a4a1f58e
名前 / ファイル | ライセンス | アクション |
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b417212d (208.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-08-13 | |||||
タイトル | ||||||
タイトル | Kinetic analysis of the activation of photoactivated adenylyl cyclase (PAC), a blue-light receptor for photomovements of Euglena | |||||
タイトル | ||||||
タイトル | Kinetic analysis of the activation of photoactivated adenylyl cyclase (PAC), a blue-light receptor for photomovements of Euglena | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
YOSHIKAWA, Shinya
× YOSHIKAWA, Shinya× SUZUKI, Takeshi× WATANABE, Masakatsu× ISEKI, Mineo |
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著者別名 |
渡辺, 正勝
× 渡辺, 正勝 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Photoactivated adenylyl cyclase (PAC) was first purified from a photosensing organelle (the paraflagellar body) of the unicellular flagellate Euglena gracilis, and is regarded as the photoreceptor for the step-up photophobic response. Here, we report the kinetic properties of photoactivation of PAC and a change in intracellular cAMP levels upon blue light irradiation. Activation of PAC was dependent both on photon fluence rate and duration of irradiation, between which reciprocity held well in the range of 2-50 μmol m-2 s-1 (total fluence of 1200 μmol m-2). Intermittent irradiation also caused activation of PAC in a photon fluence-dependent manner irrespective of cycle periods. Wavelength dependency of PAC activation showed prominent peaks in the UV-B/C, UV-A and blue regions of the spectrum. The time course of the changes in intracellular cAMP levels corresponded well with that of the step-up photophobic response. From this and the kinetic properties of PAC photoactivation, we concluded that an increase in intracellular cAMP levels evoked by photoactivation of PAC is a key event of the step-up photophobic response. | |||||
書誌情報 |
Photochemical and Photobiological Sciences en : Photochemical and Photobiological Sciences 巻 4, 号 9, p. 727-731, 発行日 2005-09 |
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出版者 | ||||||
出版者 | Royal Society of Chemistry | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1474905X | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 16121284 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1039/b417212d | |||||
関連名称 | 10.1039/b417212d | |||||
権利 | ||||||
権利情報 | © The Royal Society of Chemistry and Owner Societies 2005 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.rsc.org/AboutUs/Copyright/LicencetoPublishforjournals.asp | |||||
関連名称 | Copyright | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |