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The origin of photoactivated adenylyl cyclase (PAC), the Euglena blue-light receptor: Phylogenetic analysis of orthologues of PAC subunits from several euglenoids and trypanosome-type adenylyl cyclases from Euglena gracilis
https://ir.soken.ac.jp/records/3887
https://ir.soken.ac.jp/records/3887e65bb380-a079-4c66-82f7-1369af9c66a7
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-08-13 | |||||
| タイトル | ||||||
| タイトル | The origin of photoactivated adenylyl cyclase (PAC), the Euglena blue-light receptor: Phylogenetic analysis of orthologues of PAC subunits from several euglenoids and trypanosome-type adenylyl cyclases from Euglena gracilis | |||||
| タイトル | ||||||
| タイトル | The origin of photoactivated adenylyl cyclase (PAC), the Euglena blue-light receptor: Phylogenetic analysis of orthologues of PAC subunits from several euglenoids and trypanosome-type adenylyl cyclases from Euglena gracilis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
KOUMURA, Yoshiko
× KOUMURA, Yoshiko× SUZUKI, Takeshi× YOSHIKAWA, Shinya× WATANABE, Masakatsu× ISEKI, Mineo |
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| 著者別名 |
渡辺, 正勝
× 渡辺, 正勝 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Photoactivated adenylyl cyclase (PAC) is the blue-light receptor flavoprotein recently identified as a photoreceptor for photoavoidance of the unicellular flagellate, Euglena gracilis. To gain an insight into the evolution of this unique protein, similar sequences were searched for in several euglenoids by reverse transcriptase-polymerase chain reaction (RT-PCR) using degenerate primers. Two similar transcripts were detected in each of the four phototrophic euglenoids, Euglena stellata, Colacium sideropus, Eutreptia viridis, Eutreptiella gymnastica, and in an osmotrophic (i.e., obtaining nutrients by absorption) one, Khawkinea quartana, but not in a phagotrophic euglenoid, Petalomonas cantuscygni. Each of them seemed to be orthologous to PACα and PACβ, respectively, and had the same domain structure as PAC subunits each of which is composed of two flavin binding domains, F1 and F2, each followed by an adenylyl cyclase catalytic domain, C1 and C2, respectively. This fact implies that they constitute a functional photoactivated adenylyl cyclase like PAC. Phylogenetic analysis of the adenylyl cyclase catalytic domains revealed that they belong to a bacterial cluster, not to a trypanosomal one. In addition, two trypanosome-type adenylyl cyclases were discovered in E. gracilis. In contrast to PAC, deduced amino acid sequences of the trypanosome-type adenylyl cyclases indicated that they are integral membrane proteins with a membrane spanning region at the midpoint of them, followed by an adenylyl cyclase catalytic domain which seems cytoplasmic. Overall, we propose that PAC might have been transferred to euglenoids on the occasion of secondary endosymbiosis. | |||||
| 書誌情報 |
Photochemical and Photobiological Sciences en : Photochemical and Photobiological Sciences 巻 3, 号 6, p. 580-586, 発行日 2004-06 |
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| 出版者 | ||||||
| 出版者 | Royal Society of Chemistry | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1474905X | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 15170488 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1039/b316075k | |||||
| 関連名称 | 10.1039/b316075k | |||||
| 権利 | ||||||
| 権利情報 | © The Royal Society of Chemistry and Owner Societies 2004 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.rsc.org/AboutUs/Copyright/LicencetoPublishforjournals.asp | |||||
| 関連名称 | Copyright | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
