| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-13 |
| タイトル |
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タイトル |
Action spectra of apoptosis induction and reproductive cell death in L5178Y cells in the UV-B region |
| タイトル |
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タイトル |
Action spectra of apoptosis induction and reproductive cell death in L5178Y cells in the UV-B region |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
AOKI, Mizuho
FURUSAWA, Yoshiya
HIGASHI, Sho-ichi
WATANABE, Masakatsu
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| 著者別名 |
渡辺, 正勝
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We investigated the action spectra for the induction of apoptosis and reproductive cell death in mouse lymphoma L5178Y cells using a high-performance spectroirradiator, the Okazaki Large Spectrograph at the National Institute for Basic Biology, Okazaki. L5178Y cells were exposed to monochromatic light at different wavelengths in the UV-B and UV-A regions. The frequencies of apoptosis induction and reproductive cell death were determined by counting cells with chromatin condensation and by a semi-solidified agarose colony formation assay, respectively. The measured action spectra for the two end-points were similar. The sensitivity decreased steeply with an increase of wavelength in the UV-B region, but showed no further decrement in the UV-A region. The action spectra were slightly steeper than that for the minimum erythematic dose (MED), and were similar to the light-absorption spectrum of DNA in the UV-B region. On the other hand, in the UV-A region, the spectra for both endpoints were close to the MED, but not to DNA absorption spectra. The difference between the measured spectra and that for MED may have been caused by the absorption of the light by the skin. Differences in the time course and morphological difference of apoptosis were found between the UV-B and UV-A region. These results suggest that although DNA damage induced by UV-B light can trigger apoptosis, or lead to reproductive cell death, other damage (membrane, protein and so on) may trigger those effects in the UV-A region. |
| 書誌情報 |
Photochemical and Photobiological Sciences
en : Photochemical and Photobiological Sciences
巻 3,
号 3,
p. 268-272,
発行日 2004-03
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| 出版者 |
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出版者 |
Royal Society of Chemistry |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1474905X |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
14993943 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
http://doi.org/10.1039/b313350h |
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関連名称 |
10.1039/b313350h |
| 権利 |
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権利情報 |
© The Royal Society of Chemistry and Owner Societies 2004 |
