An action spectrum was obtained for the suppression of arylalkylamine N-acetyltransferase (NAT) activity in the two-spotted spider mite Tetranychus urticae by irradiating the mite with monochromatic lights of various wavelengths using the Okazaki Large Spectrograph at the National Institute for Basic Biology, Okazaki, Japan. Fluence-response curves were obtained for wavelengths between 300 and 650 nm by irradiating the mite for 4 h day-1. The samples were frozen after the third exposure. A negative correlation between the logarithmic fluence rate and NAT activity was detected in the range of 0.01-1 μmol m-2 s-1 for wavelengths between 300 and 500 nm and in the range of 0.1-10 μmol m-2 s-1 for wavelengths between 550 and 650 nm. The constructed action spectrum indicated that the photoreceptors mediating the circadian and/or photoperiodic systems might be UV-A- and blue-type photoreceptors with absorption peaks at 350 and 450 nm.
書誌情報
Photochemistry and Photobiology
en : Photochemistry and Photobiology