ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

  1. 010 学術雑誌論文
  2. 渡辺, 正勝 / WATANABE, Masakatsu

Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants

https://ir.soken.ac.jp/records/3923
https://ir.soken.ac.jp/records/3923
52aa8896-ca07-465c-9e99-92dce21967b8
Item type 学術雑誌論文 / Journal Article(1)
公開日 2013-08-26
タイトル
タイトル Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants
タイトル
タイトル Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 TAMURA, Kentaro

× TAMURA, Kentaro

TAMURA, Kentaro

Search repository
SHIMADA, Tomoo

× SHIMADA, Tomoo

SHIMADA, Tomoo

Search repository
ONO, Eiichiro

× ONO, Eiichiro

ONO, Eiichiro

Search repository
TANAKA, Yoshikazu

× TANAKA, Yoshikazu

TANAKA, Yoshikazu

Search repository
NAGATANI, Akira

× NAGATANI, Akira

NAGATANI, Akira

Search repository
HIGASHI, Sho-ich

× HIGASHI, Sho-ich

HIGASHI, Sho-ich

Search repository
WATANABE, Masakatsu

× WATANABE, Masakatsu

WATANABE, Masakatsu

Search repository
NISHIMURA, Mikio

× NISHIMURA, Mikio

NISHIMURA, Mikio

Search repository
HARA-NISHIMURA, Ikuko

× HARA-NISHIMURA, Ikuko

HARA-NISHIMURA, Ikuko

Search repository
著者別名 渡辺, 正勝

× 渡辺, 正勝

渡辺, 正勝

Search repository
抄録
内容記述タイプ Abstract
内容記述 Green fluorescent protein (GFP) makes it possible for organelles and protein transport pathways to be visualized in living cells. However, GFP fluorescence has not yet been observed in the vacuoles of any organs of higher plants. We found that the fluorescence of a vacuole-targeted GFP was stably observed in the vacuoles of transgenic Arabidopsis plants under dark conditions, and that the fluorescence rapidly disappeared under light conditions. The vacuolar GFP was rapidly degraded within 1 h in the light, especially blue light. An inhibitor of vacuolar type H+-ATPase, concanamycin A, and an inhibitor of papain-type cysteine proteinase, E-64d, abolished both the light-dependent disappearance of GFP fluorescence and GFP degradation in the vacuoles. An in vitro assay showed that bacterially expressed GFP was degraded by extracts of Arabidopsis cultured-cell protoplasts at an acidic pH in the light. These results suggest that blue light induced a conformational change in GFP, and the resulting GFP in the vacuole was easily degraded by vacuolar papain-type cysteine proteinase(s) under the acidic pH. The light-dependent degradation accounts for the failure to observe GFP fluorescence in the vacuoles of plant organs. Our results show that stable GFP-fluoresced vacuoles are achieved by transferring the plants from the light into the dark before inspection with a fluorescent microscope. This might eliminate a large hurdle in studies of the vacuolar-targeting machinery and the organ- and stage-specific differentiation of endomembrane systems in plants.
書誌情報 Plant Journal
en : Plant Journal

巻 35, 号 4, p. 545-555, 発行日 2003-08
出版者
出版者 Wiley-Blackwell
ISSN
収録物識別子タイプ ISSN
収録物識別子 09607412
PubMed番号
関連タイプ isIdenticalTo
識別子タイプ PMID
関連識別子 12904216
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 https://doi.org/10.1046/j.1365-313X.2003.01822.x
関連名称 10.1046/j.1365-313X.2003.01822.x
権利
権利情報 © Wiley-Blackwell
関連サイト
識別子タイプ URI
関連識別子 http://olabout.wiley.com/WileyCDA/Section/id-406074.html
関連名称 Copyright
フォーマット
内容記述タイプ Other
内容記述 application/pdf
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
戻る
0
views
See details
Views

Versions

Ver.1 2023-06-20 14:28:47.755582
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR 2.0
  • OAI-PMH JPCOAR 1.0
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3