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Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatoms
https://ir.soken.ac.jp/records/3924
https://ir.soken.ac.jp/records/3924e3fe50ae-1eba-4881-b0f5-7c8ec05947d4
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-08-26 | |||||
| タイトル | ||||||
| タイトル | Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatoms | |||||
| タイトル | ||||||
| タイトル | Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatoms | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
LEBLANC, Catherine
× LEBLANC, Catherine× FALCIATORE, Angela× WATANABE, Masakatsu× BOWLER, Chris |
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| 著者別名 |
渡辺, 正勝
× 渡辺, 正勝 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The low cell densities of diatoms and other phytoplankton in culture has precluded the use of classical RNA analysis techniques for routine studies of gene expression in large numbers of samples. This has seriously hampered studies of the basic biology of such organisms. To circumvent this problem, we have developed a high-throughput semi-quantitative RT-PCR-based protocol and used it to monitor expression of a gene encoding a fucoxanthin, chlorophyll a/c-binding protein (FCP) in the centric planktonic diatom Thalassiosira weissflogii. Analysis of FCP gene expression in dark-adapted diatom cultures revealed that mRNA levels increase 5- to 6-fold in response to white light irradiation and peak around 6 to 8 h. To determine the photoreceptors involved in this response action spectra of FCP gene expression were determined using the Okazaki large spectrograph. Responses consistent with the presence of cryptochrome-, rhodopsin- and phytochrome-type receptors could be detected. The apparent presence of phytochrome-mediated responses is of particular interest given the low fluences of red and far-red light wavelengths in the marine environment. | |||||
| 書誌情報 |
Plant Molecular Biology en : Plant Molecular Biology 巻 40, 号 6, p. 1031-1044, 発行日 1999-08 |
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| 出版者 | ||||||
| 出版者 | SpringerLink | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 01674412 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 10527427 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1023/A:1006256300969 | |||||
| 関連名称 | 10.1023/A:1006256300969 | |||||
| 権利 | ||||||
| 権利情報 | SpringerLink(The original publication is available at www.springerlink.com) | |||||
