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  1. 010 学術雑誌論文
  2. 渡辺, 正勝 / WATANABE, Masakatsu

Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatoms

https://ir.soken.ac.jp/records/3924
https://ir.soken.ac.jp/records/3924
e3fe50ae-1eba-4881-b0f5-7c8ec05947d4
Item type 学術雑誌論文 / Journal Article(1)
公開日 2013-08-26
タイトル
タイトル Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatoms
タイトル
タイトル Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatoms
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 LEBLANC, Catherine

× LEBLANC, Catherine

LEBLANC, Catherine

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FALCIATORE, Angela

× FALCIATORE, Angela

FALCIATORE, Angela

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WATANABE, Masakatsu

× WATANABE, Masakatsu

WATANABE, Masakatsu

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BOWLER, Chris

× BOWLER, Chris

BOWLER, Chris

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著者別名 渡辺, 正勝

× 渡辺, 正勝

渡辺, 正勝

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抄録
内容記述タイプ Abstract
内容記述 The low cell densities of diatoms and other phytoplankton in culture has precluded the use of classical RNA analysis techniques for routine studies of gene expression in large numbers of samples. This has seriously hampered studies of the basic biology of such organisms. To circumvent this problem, we have developed a high-throughput semi-quantitative RT-PCR-based protocol and used it to monitor expression of a gene encoding a fucoxanthin, chlorophyll a/c-binding protein (FCP) in the centric planktonic diatom Thalassiosira weissflogii. Analysis of FCP gene expression in dark-adapted diatom cultures revealed that mRNA levels increase 5- to 6-fold in response to white light irradiation and peak around 6 to 8 h. To determine the photoreceptors involved in this response action spectra of FCP gene expression were determined using the Okazaki large spectrograph. Responses consistent with the presence of cryptochrome-, rhodopsin- and phytochrome-type receptors could be detected. The apparent presence of phytochrome-mediated responses is of particular interest given the low fluences of red and far-red light wavelengths in the marine environment.
書誌情報 Plant Molecular Biology
en : Plant Molecular Biology

巻 40, 号 6, p. 1031-1044, 発行日 1999-08
出版者
出版者 SpringerLink
ISSN
収録物識別子タイプ ISSN
収録物識別子 01674412
PubMed番号
識別子タイプ PMID
関連識別子 10527427
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1023/A:1006256300969
関連名称 10.1023/A:1006256300969
権利
権利情報 SpringerLink(The original publication is available at www.springerlink.com)
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