| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-09-18 |
| タイトル |
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タイトル |
The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei |
| タイトル |
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タイトル |
The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
MARUYAMA, Eri Ohfuchi
HORI, Tetsuya
TANABE, Hideyuki
et, al.
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| 著者別名 |
田辺 , 秀之
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The spatial organization of chromatin in the nucleus contributes to genome function and is altered during the differentiation of normal and tumorigenic cells. Although nuclear actin-related proteins (Arps) have roles in the local alteration of chromatin structure, it is unclear whether they are involved in the spatial positioning of chromatin. In the interphase nucleus of vertebrate cells, gene-dense and gene-poor chromosome territories (CTs) are located in the center and periphery, respectively. We analyzed chicken DT40 cells in which Arp6 had been knocked out conditionally, and showed that the radial distribution of CTs was impaired in these knockout cells. Arp6 is an essential component of the SRCAP chromatin remodeling complex, which deposits the histone variant H2A.Z into chromatin. The redistribution of CTs was also observed in H2A.Z-deficient cells for gene-rich microchromosomes, but to lesser extent for gene-poor macrochromosomes. These results indicate that Arp6 and H2A.Z contribute to the radial distribution of CTs through different mechanisms. Microarray analysis suggested that the localization of chromatin to the nuclear periphery per se is insufficient for the repression of most genes. |
| 書誌情報 |
Journal of Cell Science
en : Journal of Cell Science
巻 125,
号 16,
p. 3739-3744,
発行日 2012-08-15
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| 出版者 |
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出版者 |
The Company of Biologists Ltd |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0021-9533 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
http://doi.org/10.1242/jcs.103903 |
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関連名称 |
10.1242/jcs.103903 |
| 権利 |
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権利情報 |
© The Company of Biologists Ltd 2013 |
