| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-09-18 |
| タイトル |
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タイトル |
Chromosomal Dynamics at the Shh Locus: Limb Bud-Specific Differential Regulation of Competence and Active Transcription |
| タイトル |
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タイトル |
Chromosomal Dynamics at the Shh Locus: Limb Bud-Specific Differential Regulation of Competence and Active Transcription |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
AMANO, Takanori
SAGAI, Tomoko
TANABE, Hideyuki
MIZUSHINA, Yoichi
NAKAZAWA, Hiromi
SHIROISHI, Toshihiko
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The expression of Sonic hedgehog (Shh) in mouse limb buds is regulated by a long-range enhancer 1 Mb upstream of the Shh promoter. We used 3D-FISH and chromosome conformation capture assays to track changes at the Shh locus and found that long-range promoter-enhancer interactions are specific to limb bud tissues competent to express Shh. However, the Shh locus loops out from its chromosome territory only in the posterior limb bud (zone of polarizing activity or ZPA), where Shh expression is active. Notably, while Shh mRNA is detected throughout the ZPA, enhancer-promoter interactions and looping out were only observed in small fractions of ZPA cells. In situ detection of nascent Shh transcripts and unstable EGFP reporters revealed that active Shh transcription is likewise only seen in a small fraction of ZPA cells. These results suggest that chromosome conformation dynamics at the Shh locus allow transient pulses of Shh transcription. |
| 書誌情報 |
Developmental Cell
en : Developmental Cell
巻 16,
号 1,
p. 47-57,
発行日 2009-01-20
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1534-5807 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.devcel.2008.11.011 |
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関連名称 |
10.1016/j.devcel.2008.11.011 |
| 権利 |
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権利情報 |
© 2009 Elsevier Inc |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
http://www.cell.com/authors |
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関連名称 |
Copyright |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
1-s2.0-S1534580708004851-main (1.3 MB)
