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Self-propagating calciferous particles detected in a human cell line Kasumi-6
https://ir.soken.ac.jp/records/3971
https://ir.soken.ac.jp/records/397167875d49-5adb-4239-8582-10d1614da9f2
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-09-19 | |||||
| タイトル | ||||||
| タイトル | Self-propagating calciferous particles detected in a human cell line Kasumi-6 | |||||
| タイトル | ||||||
| タイトル | Self-propagating calciferous particles detected in a human cell line Kasumi-6 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
HARASAWA, Ryo
× HARASAWA, Ryo× TANABE, Hideyuki× KUREMATSU, Miharu× MIZUSAWA, Hiroshi× SUZUKI, Yoshimitsu |
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| 著者別名 |
田辺, 秀之
× 田辺, 秀之 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Tiny particles were found in the medium in the presence of the human leukemia cell line Kasumi-6. The particles were separated from human cells by filtration and incubated in RPMI1640 supplemented with 10% fetal calf serum at 37 C. The particles increased in number very slowly in the liquid medium but did not reveal any biological activity. Transmission electron microscopy of the particles showed a spheroid or ovoid shape in ultrathin section. No specific polypeptides from the purified particles were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), except for bovine fetuin that adsorbed to the surface of the particles. X-ray diffractometry as well as Fourier transform infrared spectrometry suggested the particles consisted of hydroxyapatite. The mechanism of self-propagation of the hydroxyapatite particles in liquid medium is currently unknown. This type of particle has been overlooked for a long period because it is noncultivable. It will be necessary to examine its biological effects to the cultured cells. | |||||
| 書誌情報 |
In Vitro Cellular & Developmental Biology - Animal en : In Vitro Cellular & Developmental Biology - Animal 巻 42, 号 1-2, p. 13-15, 発行日 2006 |
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| 出版者 | ||||||
| 出版者 | SpringerLink | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1071-2690 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s11626-006-0005-y | |||||
| 関連名称 | 10.1007/s11626-006-0005-y | |||||
| 権利 | ||||||
| 権利情報 | SpringerLink(The original publication is available at www.springerlink.com) | |||||
