| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-09-25 |
| タイトル |
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タイトル |
Bloom helicase is involved in DNA surveillance in early S phase in vertebrate cells |
| タイトル |
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タイトル |
Bloom helicase is involved in DNA surveillance in early S phase in vertebrate cells |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
IMAMURA, Osamu
FUJITA, Kumiko
SHIMAMOTO, Akira
TANABE, Hideyuki
TAKEDA, Shunichi
FURUICHI, Yasuhiro
MATSUMOTO, Takehisa
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| 著者別名 |
田辺, 秀之
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Bloom syndrome (BS) is a recessive human genetic disorder characterized by short stature, immunodeficiency and an elevated risk of malignancy. The gene mutated in BS, BLM, encodes a RecQ-type DNA helicase. BS cells have mutator phenotypes such as hyper-recombination, chromosome instability and an increased frequency of sister chromatid exchange (SCE). To define the primary role of BLM, we generated BLM(-/-) mutants of the chicken B-cell line DT40. In addition to characteristics of BLM(-/-) cells reported previously by the other group, they are hypersensitive to genotoxic agents such as etoposide, bleomycin and 4-nitroquinoline-1-oxide and irradiation with the short wave length of UV (UVC) light, whereas they exhibit normal sensitivity to X-ray irradiation and hydroxyurea. UVC irradiation to BLM(-/-) cells during G(1) to early S phase caused chromosomal instability such as chromatid breaks and chromosomal quadriradials, leading to eventual cell death. These results suggest that BLM is involved in surveillance of base abnormalities in genomic DNA that may be encountered by replication forks in early S phase. Such surveillance would maintain genomic stability in vertebrate cells, resulting in the prevention of cellular tumorigenesis. |
| 書誌情報 |
Oncogene
en : Oncogene
巻 20,
号 10,
p. 1143-1151,
発行日 2001-03-08
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| 出版者 |
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出版者 |
Nature Publishing Group |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0950-9232 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
http://doi.org/10.1038/sj.onc.1204195 |
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関連名称 |
10.1038/sj.onc.1204195 |
| 権利 |
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権利情報 |
© 2001 Nature Publishing Group |
