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Requirement of wild-type p53 protein for maintenance of chromosomal integrity
https://ir.soken.ac.jp/records/3984
https://ir.soken.ac.jp/records/3984a8cfda9e-b381-404e-b7cc-d4e9274ee68f
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-09-25 | |||||
| タイトル | ||||||
| タイトル | Requirement of wild-type p53 protein for maintenance of chromosomal integrity | |||||
| タイトル | ||||||
| タイトル | Requirement of wild-type p53 protein for maintenance of chromosomal integrity | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
HONMA, Masamitsu
× HONMA, Masamitsu× MOMOSE, Maki× TANABE, Hideyuki× SAKAMOTO, Hiroko× YU, Yongjia× LITTLE, John B× SOFUNI, Toshio× HAYASHI, Makoto |
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| 著者別名 |
田辺, 秀之
× 田辺, 秀之 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Chromosomal double-strand breaks (DSBs) occurring in mammalian cells can initiate genomic instability, and their misrepairs result in chromosomal deletion, amplification, and translocation, common findings in human tumors. The tumor-suppressor protein p53 is involved in maintaining genomic stability. In this study, we demonstrate that the deficiency of wild-type p53 protein may allow unrepaired DSBs to initiate chromosomal instability. The human lymphoblastoid cell line TK6-E6 was established by transfection with human papilloma virus 16 (HPV16) E6 cDNA into parental TK6 cells via a retroviral vector. Abrogation of p53 function by E6 resulted in an increase in the spontaneous mutation frequencies at the heterozygous thymidine kinase (TK) locus but not at the hemizygous hypoxanthine phosphoribosyl transferase (HPRT) locus. Almost all TK-deficient mutants from TK6-E6 cells exhibited loss of heterozygosity (LOH) with the hemizygous TK allele. LOH analysis with microsatellite loci spanning the long arm of chromosome 17, which harbors the TK locus, showed that LOH extended over half of 17q toward the terminal end. Cytogenetic analysis of LOH mutants by chromosome painting indicated a mosaic of chromosomal aberrations involving chromosome 17, in which partial chromosome deletions, amplifications, and multiple translocations appeared heterogeneously in a single mutant. We speculate that spontaneous DSBs trigger the breakage-fusion bridge cycle leading to such multiple chromosome aberrations. In contrast, no chromosomal alterations were observed in TK-deficient mutants from TK6-20C cells expressing wild-type p53. In wild-type p53 cells, spontaneous DSBs appear to be promptly repaired through recombination between homologous chromosomes. These results support a model in which p53 protein contributes to the maintenance of genomic integrity through recombinational repair. | |||||
| 書誌情報 |
Molecular Carcinogenesis en : Molecular Carcinogenesis 巻 28, 号 4, p. 203-214, 発行日 2000-08 |
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| 出版者 | ||||||
| 出版者 | Wiley-Blackwell | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0899-1987 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1002/1098-2744(200008)28:4<203::AID-MC3>3.0.CO;2-1 | |||||
| 関連名称 | 10.1002/1098-2744(200008)28:4<203::AID-MC3>3.0.CO;2-1 | |||||
| 権利 | ||||||
| 権利情報 | © 2000 Wiley-Liss, Inc | |||||
