{"_buckets": {"deposit": "667eb816-fd54-4423-beb0-0cfaa6b436b8"}, "_deposit": {"created_by": 21, "id": "4009", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "4009"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00004009", "sets": ["283"]}, "author_link": ["345", "346", "2170", "1818"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-04-08", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "1120", "bibliographicPageStart": "1108", "bibliographicVolumeNumber": "4", "bibliographic_titles": [{"bibliographic_title": "The ISME Journal "}, {"bibliographic_title": "The ISME Journal ", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "渡辺, 正勝"}], "nameIdentifiers": [{"nameIdentifier": "345", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000040124226", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "40124226", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=40124226"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Petalomonas sphagnophila is a poorly studied plastid-lacking euglenid flagellate living in Sphagnum-dominated peatlands. Here we present a broad-ranging microscopic, molecular and microspectrophotometric analysis of uncultured P. sphagnophila collected from four field locations in Nova Scotia, Canada. Consistent with its morphological characteristics, 18S ribosomal DNA (rDNA) phylogenies indicate that P. sphagnophila is specifically related to Petalomonas cantuscygni, the only other Petalomonas species sequenced to date. One of the peculiar characteristics of P. sphagnophila is the presence of several green-pigmented particles ~5 μm in diameter in its cytoplasm, which a previously published study suggested to be cyanobacterial endosymbionts. New data presented here, however, suggest that the green intracellular body may not be a cyanobacterium but rather an uncharacterized prokaryote yet to be identified by molecular sequencing. 16S rDNA library sequencing and fluorescence in situ hybridizations show that P. sphagnophila also harbors several other endobionts, including bacteria that represent five novel genus-level groups (one firmicute and four different proteobacteria). 16S rDNA phylogenies suggest that three of these endobionts are related to obligate intracellular bacteria such as Rickettsiales and Coxiella, while the others are related to the Daphnia pathogen Spirobacillus cienkowskii or belong to the Thermoactinomycetaceae. TEM, 16S rDNA library sequencing and a battery of PCR experiments show that the presence of the five P. sphagnophila endobionts varies markedly among the four geographic collections and even among individuals collected from the same location but at different time points. Our study adds significantly to the growing evidence for complex and dynamic protist–bacterial associations in nature.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nature Publishing Group"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1038/ismej.2010.40"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1038/ismej.2010.40", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2013 International Society for Microbial Ecology"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1751-7362", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KIM, Eunsoo"}], "nameIdentifiers": [{"nameIdentifier": "2170", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "WATANABE, Masakatsu"}], "nameIdentifiers": [{"nameIdentifier": "346", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "and , Others"}], "nameIdentifiers": [{"nameIdentifier": "1818", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Complex array of endobionts in Petalomonas sphagnophila, a large heterotrophic euglenid protist from Sphagnum-dominated peatlands", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Complex array of endobionts in Petalomonas sphagnophila, a large heterotrophic euglenid protist from Sphagnum-dominated peatlands"}, {"subitem_title": "Complex array of endobionts in Petalomonas sphagnophila, a large heterotrophic euglenid protist from Sphagnum-dominated peatlands", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["109", "283"], "permalink_uri": "https://ir.soken.ac.jp/records/4009", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-10-11"}, "publish_date": "2013-10-11", "publish_status": "0", "recid": "4009", "relation": {}, "relation_version_is_last": true, "title": ["Complex array of endobionts in Petalomonas sphagnophila, a large heterotrophic euglenid protist from Sphagnum-dominated peatlands"], "weko_shared_id": 21}