WEKO3
アイテム
Electrophoretic Investigations of the Acid Conformational Change of α-Lactalbumin
https://ir.soken.ac.jp/records/4111
https://ir.soken.ac.jp/records/4111741c6da1-d09e-4e4e-bca9-f429b6990d73
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-12-24 | |||||
| タイトル | ||||||
| タイトル | Electrophoretic Investigations of the Acid Conformational Change of α-Lactalbumin | |||||
| タイトル | ||||||
| タイトル | Electrophoretic Investigations of the Acid Conformational Change of α-Lactalbumin | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
KUWAJIMA, Kunihiro
× KUWAJIMA, Kunihiro× NITTA, Katsutoshi× SUGAI, Shintaro |
|||||
| 著者別名 |
桑島, 邦博
× 桑島, 邦博 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In order to clarify how the electrophoretic behavior reflects the conformational transition of globular proteins, moving boundary electrophoresis was applied to analysis of the acid conformational change of α-lactalbumin. The appearance of only a single electrophoretic boundary in the transition region of the protein suggests a very rapid transition with a half-time estimated to be smaller than 7 min on the basis of the theory of isomerizing systems in electrophoresis. The transition is clearly reflected in the dependence of the mobility on the protein net charge, which shows a sigmoidal curve closely similar to that obtained by a LinderstrømLang pH-titration plot for the carboxyl groups of α-lactalbumin. It was also concluded from the transition curves that the acidfication does not result in complete unfolding, but that a compact structure is maintained in the acidic region with an apparently expanded form as compared to the native state of the protein. All results obtained by electrophoresis were also supported by the results of pH-jump studies, analytical gel chromatography, and CD measurements. | |||||
| 書誌情報 |
Journal of Biochemistry en : Journal of Biochemistry 巻 78, 号 1, p. 205-211, 発行日 1975 |
|||||
| 出版者 | ||||||
| 出版者 | Oxford University Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0021924X | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 376 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1093/oxfordjournals.jbchem.a130885 | |||||
| 関連名称 | 10.1093/oxfordjournals.jbchem.a130885 | |||||
| 権利 | ||||||
| 権利情報 | © The Japanese Biochemical Society | |||||
