WEKO3
アイテム
Thermodynamic characterization of partially denatured states in denaturation process of bovine -lactalbumin by inorganic denaturants
https://ir.soken.ac.jp/records/4116
https://ir.soken.ac.jp/records/4116a02a55f7-cb04-4565-b58b-cfcbbd1fde32
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-12-24 | |||||
| タイトル | ||||||
| タイトル | Thermodynamic characterization of partially denatured states in denaturation process of bovine -lactalbumin by inorganic denaturants | |||||
| タイトル | ||||||
| タイトル | Thermodynamic characterization of partially denatured states in denaturation process of bovine -lactalbumin by inorganic denaturants | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
MARUYAMA, Satoshi
× MARUYAMA, Satoshi× KUWAJIMA, Kunihiro× NITTA, Katsutoshi× SUGAI, Shintaro |
|||||
| 著者別名 |
桑島, 邦博
× 桑島, 邦博 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In an attempt to understand the specific effect of inorganic protein denaturants, lithium cation and perchlorate anion, upon the molecular conformation of bovine α-lactalbumin and to characterize the denatured states of the protein and the denaturation processes, themodynamic studies on the reversible unfolding of the protein in the presence of lithium chloride, lithium perchlorate and sodium perchlorate were made by means of circular dichroism and ultraviolet absorption measurements. The denaturation reaction caused by lithium chloride was found to take place in a three-state type, while that caused by the two perchlorates in a two-state type. The latter produces the same denatured state as the acid does on the protein, the state where the helical structures remain unchanged. The former produces two kinds of the denatured state, one being a less unfolded state than the acid denatured one and the other a fully unfolded state which is identical with the finally denatured state induced by organic denaturants such as guanidinium chloride, guanidinium thiocyanate and urea. | |||||
| 書誌情報 |
Biochimica et Biophysica Acta en : Biochimica et Biophysica Acta 巻 494, 号 2, p. 343-353, 発行日 1977-10-26 |
|||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006-3002 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/0005-2795(77)90164-7 | |||||
| 関連名称 | 10.1016/0005-2795(77)90164-7 | |||||
| 権利 | ||||||
| 権利情報 | © 1977 Published by Elsevier B.V. | |||||
