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  1. 010 学術雑誌論文
  2. 桑島, 邦博 / KUWAJIMA, Kunihiro

Thermodynamic characterization of partially denatured states in denaturation process of bovine -lactalbumin by inorganic denaturants

https://ir.soken.ac.jp/records/4116
https://ir.soken.ac.jp/records/4116
a02a55f7-cb04-4565-b58b-cfcbbd1fde32
Item type 学術雑誌論文 / Journal Article(1)
公開日 2013-12-24
タイトル
タイトル Thermodynamic characterization of partially denatured states in denaturation process of bovine -lactalbumin by inorganic denaturants
タイトル
言語 en
タイトル Thermodynamic characterization of partially denatured states in denaturation process of bovine -lactalbumin by inorganic denaturants
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 MARUYAMA, Satoshi

× MARUYAMA, Satoshi

WEKO 2368

MARUYAMA, Satoshi

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KUWAJIMA, Kunihiro

× KUWAJIMA, Kunihiro

WEKO 2361

KUWAJIMA, Kunihiro

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NITTA, Katsutoshi

× NITTA, Katsutoshi

WEKO 2362

NITTA, Katsutoshi

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SUGAI, Shintaro

× SUGAI, Shintaro

WEKO 2363

SUGAI, Shintaro

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著者別名 桑島, 邦博

× 桑島, 邦博

WEKO 2364
NRID 1000070091444
e-Rad 70091444

桑島, 邦博

Search repository
抄録
内容記述タイプ Abstract
内容記述 In an attempt to understand the specific effect of inorganic protein denaturants, lithium cation and perchlorate anion, upon the molecular conformation of bovine α-lactalbumin and to characterize the denatured states of the protein and the denaturation processes, themodynamic studies on the reversible unfolding of the protein in the presence of lithium chloride, lithium perchlorate and sodium perchlorate were made by means of circular dichroism and ultraviolet absorption measurements. The denaturation reaction caused by lithium chloride was found to take place in a three-state type, while that caused by the two perchlorates in a two-state type. The latter produces the same denatured state as the acid does on the protein, the state where the helical structures remain unchanged. The former produces two kinds of the denatured state, one being a less unfolded state than the acid denatured one and the other a fully unfolded state which is identical with the finally denatured state induced by organic denaturants such as guanidinium chloride, guanidinium thiocyanate and urea.
書誌情報 Biochimica et Biophysica Acta
en : Biochimica et Biophysica Acta

巻 494, 号 2, p. 343-353, 発行日 1977-10-26
出版者
出版者 Elsevier
ISSN
収録物識別子タイプ ISSN
収録物識別子 0006-3002
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/0005-2795(77)90164-7
関連名称 10.1016/0005-2795(77)90164-7
権利
権利情報 © 1977 Published by Elsevier B.V.
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