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Intramolecular perturbation of tryptophans induced by the protonation of ionizable groups in goat -lactalbumin
https://ir.soken.ac.jp/records/4121
https://ir.soken.ac.jp/records/4121059bc645-b3a0-46d4-b407-7984ba5b1e83
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-12-25 | |||||
| タイトル | ||||||
| タイトル | Intramolecular perturbation of tryptophans induced by the protonation of ionizable groups in goat -lactalbumin | |||||
| タイトル | ||||||
| タイトル | Intramolecular perturbation of tryptophans induced by the protonation of ionizable groups in goat -lactalbumin | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
KUWAJIMA, Kunihiro
× KUWAJIMA, Kunihiro× NITTA, Katsutoshi× SUGAI, Shintaro |
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| 著者別名 |
桑島, 邦博
× 桑島, 邦博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Goat α-lactalbumin shows two spectral changes at acid pH. One of these corresponds to the acid denaturation, while the other has been regarded as a result of the intramolecular perturbation of tryptophans induced by the protonation of ionizable groups in the native molecule. In order to distinguish between these spectral changes and to investigate the perturbation caused by the ionizable groups, the absorption changes with a change in pH have been followed by the stopped-flow pH-jump method. Only the acid denaturation can be observed kinetically while the perturbation is too rapid to follow by the kinetic method. Extrapolations of the time-dependent absorption changes to zero time in the unfolding and refolding pH jumps give the apparent titration curves of the ionizable groups that participate in the perturbations in the native and in the acid-denatured molecule. The perturbation of the native protein is induced by the protonation of at least two ionizable groups, while the acid-denatured molecule does not show the perturbation because of the loss of the unique spatial arrangement of the ionizable groups around tryptophans. The results of circular dichroism measurements and the comparison with known results of the charge perturbation of lysozyme are also discussed. | |||||
| 書誌情報 |
Biochimica et Biophysica Acta en : Biochimica et Biophysica Acta 巻 623, 号 2, p. 389-401, 発行日 1980-06-26 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006-3002 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 7397222 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/0005-2795(80)90268-8 | |||||
| 関連名称 | 10.1016/0005-2795(80)90268-8 | |||||
| 権利 | ||||||
| 権利情報 | © 1980 Published by Elsevier B.V. | |||||
