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  1. 010 学術雑誌論文
  2. 桑島, 邦博 / KUWAJIMA, Kunihiro

Intramolecular perturbation of tryptophans induced by the protonation of ionizable groups in goat -lactalbumin

https://ir.soken.ac.jp/records/4121
https://ir.soken.ac.jp/records/4121
059bc645-b3a0-46d4-b407-7984ba5b1e83
Item type 学術雑誌論文 / Journal Article(1)
公開日 2013-12-25
タイトル
タイトル Intramolecular perturbation of tryptophans induced by the protonation of ionizable groups in goat -lactalbumin
タイトル
タイトル Intramolecular perturbation of tryptophans induced by the protonation of ionizable groups in goat -lactalbumin
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 KUWAJIMA, Kunihiro

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KUWAJIMA, Kunihiro

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NITTA, Katsutoshi

× NITTA, Katsutoshi

NITTA, Katsutoshi

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SUGAI, Shintaro

× SUGAI, Shintaro

SUGAI, Shintaro

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著者別名 桑島, 邦博

× 桑島, 邦博

桑島, 邦博

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抄録
内容記述タイプ Abstract
内容記述 Goat α-lactalbumin shows two spectral changes at acid pH. One of these corresponds to the acid denaturation, while the other has been regarded as a result of the intramolecular perturbation of tryptophans induced by the protonation of ionizable groups in the native molecule. In order to distinguish between these spectral changes and to investigate the perturbation caused by the ionizable groups, the absorption changes with a change in pH have been followed by the stopped-flow pH-jump method. Only the acid denaturation can be observed kinetically while the perturbation is too rapid to follow by the kinetic method. Extrapolations of the time-dependent absorption changes to zero time in the unfolding and refolding pH jumps give the apparent titration curves of the ionizable groups that participate in the perturbations in the native and in the acid-denatured molecule. The perturbation of the native protein is induced by the protonation of at least two ionizable groups, while the acid-denatured molecule does not show the perturbation because of the loss of the unique spatial arrangement of the ionizable groups around tryptophans. The results of circular dichroism measurements and the comparison with known results of the charge perturbation of lysozyme are also discussed.
書誌情報 Biochimica et Biophysica Acta
en : Biochimica et Biophysica Acta

巻 623, 号 2, p. 389-401, 発行日 1980-06-26
出版者
出版者 Elsevier
ISSN
収録物識別子タイプ ISSN
収録物識別子 0006-3002
PubMed番号
識別子タイプ PMID
関連識別子 7397222
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/0005-2795(80)90268-8
関連名称 10.1016/0005-2795(80)90268-8
権利
権利情報 © 1980 Published by Elsevier B.V.
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