| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-01-09 |
| タイトル |
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|
タイトル |
Binding Strength of Calcium Ion to Bovine α-Lactalbnmin |
| タイトル |
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タイトル |
Binding Strength of Calcium Ion to Bovine α-Lactalbnmin |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
HAMANO, Masaki
NITTA, Katsutoshi
KUWAJIMA, Kunihiro
SUGAI, Shintaro
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| 著者別名 |
桑島, 邦博
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
A Ca2+-sensitive electrode was used for determination of the binding strength of Ca2+ to bovine α-lactalbumin in 60 mM Tris buffer (pH 7.8–8.5) in the presence of various concentrations of NaCl. The dependence of the apparent binding constant on the concentration of NaCl was consistent with competitive binding of Ca2+ and Na+, and the binding constants of Ca2+ and Na+ were found to be 2.2 (±0.5)× 107M−1 and 99 (±33) M−1, respectively, at 37°C and pH 8.0. The temperature dependence of the binding constant of Ca2+ was examined between 30 and 45°C; extrapolation of the dependence led to a binding constant of ˜1 × 107 M−1 at pH 8.4 and 25°C. The electrostatic contribution and conformational effect of the protein were also taken into consideration, and the intrinsic binding constant of Ca2+ to native a-lactalbumin was calculated to be (1.2–1.5) × 1010 M−1 at 37°C and pH 8.0. |
| 書誌情報 |
Journal of Biochemistry
en : Journal of Biochemistry
巻 100,
号 6,
p. 1617-1622,
発行日 1986
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| 出版者 |
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出版者 |
Oxford University Press |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0021924X |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/oxfordjournals.jbchem.a121869 |
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関連名称 |
10.1093/oxfordjournals.jbchem.a121869 |
| 権利 |
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権利情報 |
© 1986, the Japanese Biochemical Society |
