| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-01-17 |
| タイトル |
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|
タイトル |
Effects of amino acid substitutions in the hydrophobic core of α-lactalbumin on the stability of the molten globule state |
| タイトル |
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タイトル |
Effects of amino acid substitutions in the hydrophobic core of α-lactalbumin on the stability of the molten globule state |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
UCHIYAMA, Hidefumi
PEREZ-PRAT, Eva M
WATANABE, Kimitsuna
KUMAGAI, Izumi
KUWAJIMA, Kunihiro
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| 著者別名 |
桑島, 邦博
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Five mutant α–lactalbumins, with one or two amino acid substitution(s) in the B helix, were engineered to examine the relation between the stability of the molten globule state and the hydrophobicity of these amino acids. The mutation sites (Thr29, Ala30 and Thr33) have been chosen on the basis of comparison of the amino acid sequences of goat, bovine and gunea pig α–lactalbumin, in which the guinea pig protein shows a remarkably more stable molten globule than the other proteins. The recombinant proteins were expressed Escherichia coli and then purified and refolded efficiently to produce the active proteins. The stability of the molten globule state of these engineered proteins has been investigated by urea–induced unfolding transition under an acidic condition (pH 2.0), where the molten globule state is stable in the absence of urea. The results show that the molten globule state is stabilized by the amino acid substitutions which raise the hydrophobicity of the residues, suggesting that the hydrophobic core in a globular protein plays an important role in the stability of the molten globule state. The change in stabilization free energy of the molten globule state caused by each amino acid substitution has been evaluated, and molecular mechanisms of stabilization of the molten globule state are discussed. |
| 書誌情報 |
Protein Engineering, Design and Selection
en : Protein Engineering, Design and Selection
巻 8,
号 11,
p. 1153-1161,
発行日 1995
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| 出版者 |
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出版者 |
Oxford University Press |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1741-0126 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
8819981 |
| DOI |
|
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1093/protein/8.11.1153 |
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関連名称 |
10.1093/protein/8.11.1153 |
| 権利 |
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|
権利情報 |
© Oxford University Press |
