{"_buckets": {"deposit": "34eb2054-95ae-4804-9c70-a55fa7de072d"}, "_deposit": {"created_by": 21, "id": "4275", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "4275"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00004275", "sets": ["323"]}, "author_link": ["2364", "2361", "2407", "2489"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1997", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "541", "bibliographicPageStart": "534", "bibliographicVolumeNumber": "121", "bibliographic_titles": [{"bibliographic_title": "Journal of Biochemistry"}, {"bibliographic_title": "Journal of Biochemistry", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "桑島, 邦博"}], "nameIdentifiers": [{"nameIdentifier": "2364", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000070091444", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "70091444", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70091444"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "α-Lactalbumin in which all the disulfide bonds are fully reduced (RLA) is known to bind strongly to the chaperonin GroEL. Although RLA is more unfolded than the native state and the molten globule state of α-lactalbumin, the CD spectrum of RLA in the far-UV region shows that RLA is not fully unfolded but has an appreciable amount of secondary structure. To investigate whether the secondary structure elements present in RLA are responsible for the recognition of RLA by GroEL or not, we have examined the hydrogen-exchange kinetics of RLA in the presence and absence of GroEL. Our results show that the hydrogen-exchange kinetics of RLA bound to GroEL is identical to that of free RLA. This implies that the secondary structure elements in RLA are not important for the recognition by GroEL, but the unstructured parts of RLA that are not relevant to the stability of the secondary structure provide strong recognition sites of RLA. ", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Oxford University Press "}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1093/oxfordjournals.jbchem.a021619"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1093/oxfordjournals.jbchem.a021619 ", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© The Japanese Biochemical Society"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0021-924X", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "OKAZAKI, Akira "}], "nameIdentifiers": [{"nameIdentifier": "2407", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KATSUMATA, Kumiko"}], "nameIdentifiers": [{"nameIdentifier": "2489", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KUWAJIMA, Kunihiro "}], "nameIdentifiers": [{"nameIdentifier": "2361", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Hydrogen-Exchange Kinetics of Reduced α-Lactalbumin Bound to the Chaperonin GroEL", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Hydrogen-Exchange Kinetics of Reduced α-Lactalbumin Bound to the Chaperonin GroEL"}, {"subitem_title": "Hydrogen-Exchange Kinetics of Reduced α-Lactalbumin Bound to the Chaperonin GroEL", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["323"], "permalink_uri": "https://ir.soken.ac.jp/records/4275", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-03-10"}, "publish_date": "2014-03-10", "publish_status": "0", "recid": "4275", "relation": {}, "relation_version_is_last": true, "title": ["Hydrogen-Exchange Kinetics of Reduced α-Lactalbumin Bound to the Chaperonin GroEL"], "weko_shared_id": 21}