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Hydrogen-Exchange Kinetics of Reduced α-Lactalbumin Bound to the Chaperonin GroEL
https://ir.soken.ac.jp/records/4275
https://ir.soken.ac.jp/records/4275de451729-66b6-4b43-93e5-721107886a6f
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-03-10 | |||||
| タイトル | ||||||
| タイトル | Hydrogen-Exchange Kinetics of Reduced α-Lactalbumin Bound to the Chaperonin GroEL | |||||
| タイトル | ||||||
| タイトル | Hydrogen-Exchange Kinetics of Reduced α-Lactalbumin Bound to the Chaperonin GroEL | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
OKAZAKI, Akira
× OKAZAKI, Akira× KATSUMATA, Kumiko× KUWAJIMA, Kunihiro |
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| 著者別名 |
桑島, 邦博
× 桑島, 邦博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | α-Lactalbumin in which all the disulfide bonds are fully reduced (RLA) is known to bind strongly to the chaperonin GroEL. Although RLA is more unfolded than the native state and the molten globule state of α-lactalbumin, the CD spectrum of RLA in the far-UV region shows that RLA is not fully unfolded but has an appreciable amount of secondary structure. To investigate whether the secondary structure elements present in RLA are responsible for the recognition of RLA by GroEL or not, we have examined the hydrogen-exchange kinetics of RLA in the presence and absence of GroEL. Our results show that the hydrogen-exchange kinetics of RLA bound to GroEL is identical to that of free RLA. This implies that the secondary structure elements in RLA are not important for the recognition by GroEL, but the unstructured parts of RLA that are not relevant to the stability of the secondary structure provide strong recognition sites of RLA. | |||||
| 書誌情報 |
Journal of Biochemistry en : Journal of Biochemistry 巻 121, 号 3, p. 534-541, 発行日 1997 |
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| 出版者 | ||||||
| 出版者 | Oxford University Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0021-924X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1093/oxfordjournals.jbchem.a021619 | |||||
| 関連名称 | 10.1093/oxfordjournals.jbchem.a021619 | |||||
| 権利 | ||||||
| 権利情報 | © The Japanese Biochemical Society | |||||
