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Hydrophilic Residues at the Apical Domain of GroEL Contribute to GroES Binding but Attenuate Polypeptide Binding
https://ir.soken.ac.jp/records/4292
https://ir.soken.ac.jp/records/4292b2a0ccdc-d923-45b7-ae97-6846e9e6ff17
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-03-13 | |||||
| タイトル | ||||||
| タイトル | Hydrophilic Residues at the Apical Domain of GroEL Contribute to GroES Binding but Attenuate Polypeptide Binding | |||||
| タイトル | ||||||
| タイトル | Hydrophilic Residues at the Apical Domain of GroEL Contribute to GroES Binding but Attenuate Polypeptide Binding | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
MOTOJIMA, Fumihiro
× MOTOJIMA, Fumihiro× KUWAJIMA, Kunihiro× et, al. |
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| 著者別名 |
桑島, 邦博
× 桑島, 邦博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The GroES binding site at the apical domain of GroEL, mostly consisting of hydrophobic residues, overlaps largely with the substrate polypeptide binding site. Essential contribution of hydrophobic interaction to the binding of both GroES and polypeptide was exemplified by the mutant GroEL(L237Q) which lost the ability to bind either of them. The binding site, however, contains three hydrophilic residues, E238, T261, and N265. For GroES binding, N265 is essential since GroEL(N265A) is unable to bind GroES. E238 contributes to rapid GroES binding to GroEL because GroEL(E238A) is extremely sluggish in GroES binding. Polypeptide binding was not impaired by any mutations of E238A, T261A, and N265A. Rather, these mutants, especially GroEL(N265A), showed stronger polypeptide binding affinity than wild-type GroEL. Thus, these hydrophilic residues have a dual role; they help GroES binding on one hand but attenuate polypeptide binding on the other hand. | |||||
| 書誌情報 |
Biochemical and Biophysical Research Communications en : Biochemical and Biophysical Research Communications 巻 267, 号 3, p. 842-849, 発行日 2000-01-27 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006-291X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1006/bbrc.1999.2020 | |||||
| 関連名称 | 10.1006/bbrc.1999.2020 | |||||
| 権利 | ||||||
| 権利情報 | © 2000 Academic Press | |||||
