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  1. 010 学術雑誌論文
  2. 桑島, 邦博 / KUWAJIMA, Kunihiro

Folding mechanism of canine milk lysozyme studied by circular dichroism and fluorescence spectroscopy

https://ir.soken.ac.jp/records/4308
https://ir.soken.ac.jp/records/4308
29db16fd-208c-497e-9940-5d12d5cc27ba
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-03-18
タイトル
タイトル Folding mechanism of canine milk lysozyme studied by circular dichroism and fluorescence spectroscopy
タイトル
タイトル Folding mechanism of canine milk lysozyme studied by circular dichroism and fluorescence spectroscopy
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 NAKAO, Masaharu

× NAKAO, Masaharu

NAKAO, Masaharu

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KUWAJIMA, Kunihiro

× KUWAJIMA, Kunihiro

KUWAJIMA, Kunihiro

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et, al.

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et, al.

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著者別名 桑島, 邦博

× 桑島, 邦博

桑島, 邦博

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抄録
内容記述タイプ Abstract
内容記述 We have studied the guanidine hydrochloride-induced equilibrium unfolding and the kinetics of refolding of canine milk lysozyme by circular dichroism and fluorescence spectroscopy. The thermodynamic analysis of the equilibrium unfolding measured by circular dichroism and fluorescence has shown that unfolding is represented by a three-state mechanism and that the intermediate state of canine milk lysozyme is remarkably more stable than the intermediates observed in other lysozyme and α-lactalbumin. In the kinetic refolding of this protein, there are at least two kinetic intermediates; a burst-phase intermediate accumulated within the dead time (4 ms) of the measurement and an intermediate that has been observed during the kinetics with a rate constant of 10–20 s-1 after the burst phase. This result is apparently in contrast with those previously observed in the kinetic refolding of α-lactalbumin and equine lysozyme that show only the burst-phase intermediate. The relationship between the extraordinarily stable equilibrium molten globule and the kinetic folding intermediates will be discussed.
書誌情報 Spectroscopy: An International Journal
en : Spectroscopy: An International Journal

巻 17, 号 2-3, p. 183-193, 発行日 2003
出版者
出版者 IOS Press
ISSN
収録物識別子タイプ ISSN
収録物識別子 0712-4813
権利
権利情報 ©2003 IOS Press
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