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Folding mechanism of canine milk lysozyme studied by circular dichroism and fluorescence spectroscopy
https://ir.soken.ac.jp/records/4308
https://ir.soken.ac.jp/records/430829db16fd-208c-497e-9940-5d12d5cc27ba
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2014-03-18 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Folding mechanism of canine milk lysozyme studied by circular dichroism and fluorescence spectroscopy | |||||||||||
| タイトル | ||||||||||||
| タイトル | Folding mechanism of canine milk lysozyme studied by circular dichroism and fluorescence spectroscopy | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | metadata only access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||
| 著者 |
NAKAO, Masaharu
× NAKAO, Masaharu
× KUWAJIMA, Kunihiro
× et, al.
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| 著者別名 |
桑島, 邦博
× 桑島, 邦博
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | We have studied the guanidine hydrochloride-induced equilibrium unfolding and the kinetics of refolding of canine milk lysozyme by circular dichroism and fluorescence spectroscopy. The thermodynamic analysis of the equilibrium unfolding measured by circular dichroism and fluorescence has shown that unfolding is represented by a three-state mechanism and that the intermediate state of canine milk lysozyme is remarkably more stable than the intermediates observed in other lysozyme and α-lactalbumin. In the kinetic refolding of this protein, there are at least two kinetic intermediates; a burst-phase intermediate accumulated within the dead time (4 ms) of the measurement and an intermediate that has been observed during the kinetics with a rate constant of 10–20 s-1 after the burst phase. This result is apparently in contrast with those previously observed in the kinetic refolding of α-lactalbumin and equine lysozyme that show only the burst-phase intermediate. The relationship between the extraordinarily stable equilibrium molten globule and the kinetic folding intermediates will be discussed. | |||||||||||
| 書誌情報 |
Spectroscopy: An International Journal en : Spectroscopy: An International Journal 巻 17, 号 2-3, p. 183-193, 発行日 2003 |
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| 出版者 | ||||||||||||
| 出版者 | IOS Press | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0712-4813 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | ©2003 IOS Press | |||||||||||
