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  1. 010 学術雑誌論文
  2. 桑島, 邦博 / KUWAJIMA, Kunihiro

Multiple parallel-pathway folding of proline-free Staphylococcal nuclease

https://ir.soken.ac.jp/records/4309
https://ir.soken.ac.jp/records/4309
d971649e-dbff-4e3e-82db-72a76e1cfe04
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-03-18
タイトル
タイトル Multiple parallel-pathway folding of proline-free Staphylococcal nuclease
タイトル
タイトル Multiple parallel-pathway folding of proline-free Staphylococcal nuclease
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 KAMAGATA, Kiyoto

× KAMAGATA, Kiyoto

KAMAGATA, Kiyoto

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SAWANO, Yoriko

× SAWANO, Yoriko

SAWANO, Yoriko

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TANOKURA, Masaru

× TANOKURA, Masaru

TANOKURA, Masaru

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KUWAJIMA, Kunihiro

× KUWAJIMA, Kunihiro

KUWAJIMA, Kunihiro

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著者別名 桑島, 邦博

× 桑島, 邦博

桑島, 邦博

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抄録
内容記述タイプ Abstract
内容記述 When a protein exhibits complex kinetics of refolding, we often ascribe the complexity to slow isomerization events in the denatured protein, such as cis/trans isomerization of peptidyl prolyl bonds. Does the complex folding kinetics arise only from this well-known reason? Here, we have investigated the refolding of a proline-free variant of staphylococcal nuclease by stopped-flow, double-jump techniques, to examine the folding reactions without the slow prolyl isomerizations. As a result, the protein folds into the native state along at least two accessible parallel pathways, starting from a macroscopically single denatured-state ensemble. The presence of intermediates on the individual folding pathways has revealed the existence of multiple parallel pathways, and is characterized by multi-exponential folding kinetics with a lag phase. Therefore, a “single” amino acid sequence can fold along the multiple parallel pathways. This observation in staphylococcal nuclease suggests that the multiple folding may be more general than we have expected, because the multiple parallel-pathway folding cannot be excluded from proteins that show simpler kinetics.
書誌情報 Journal of Molecular Biology
en : Journal of Molecular Biology

巻 332, 号 5, p. 1143-1153, 発行日 2003-10-03
出版者
出版者 Elsevier
ISSN
収録物識別子タイプ ISSN
収録物識別子 0022-2836
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.jmb.2003.07.002
関連名称 10.1016/j.jmb.2003.07.002
権利
権利情報 © 2003 Elsevier Science Ltd.
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