| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-03-18 |
| タイトル |
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タイトル |
Multiple parallel-pathway folding of proline-free Staphylococcal nuclease |
| タイトル |
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タイトル |
Multiple parallel-pathway folding of proline-free Staphylococcal nuclease |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
KAMAGATA, Kiyoto
SAWANO, Yoriko
TANOKURA, Masaru
KUWAJIMA, Kunihiro
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| 著者別名 |
桑島, 邦博
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
When a protein exhibits complex kinetics of refolding, we often ascribe the complexity to slow isomerization events in the denatured protein, such as cis/trans isomerization of peptidyl prolyl bonds. Does the complex folding kinetics arise only from this well-known reason? Here, we have investigated the refolding of a proline-free variant of staphylococcal nuclease by stopped-flow, double-jump techniques, to examine the folding reactions without the slow prolyl isomerizations. As a result, the protein folds into the native state along at least two accessible parallel pathways, starting from a macroscopically single denatured-state ensemble. The presence of intermediates on the individual folding pathways has revealed the existence of multiple parallel pathways, and is characterized by multi-exponential folding kinetics with a lag phase. Therefore, a “single” amino acid sequence can fold along the multiple parallel pathways. This observation in staphylococcal nuclease suggests that the multiple folding may be more general than we have expected, because the multiple parallel-pathway folding cannot be excluded from proteins that show simpler kinetics. |
| 書誌情報 |
Journal of Molecular Biology
en : Journal of Molecular Biology
巻 332,
号 5,
p. 1143-1153,
発行日 2003-10-03
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0022-2836 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.jmb.2003.07.002 |
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関連名称 |
10.1016/j.jmb.2003.07.002 |
| 権利 |
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権利情報 |
© 2003 Elsevier Science Ltd. |
