ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "091b24ab-f937-4355-a436-8ddfd74cab9b"}, "_deposit": {"created_by": 21, "id": "4318", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "4318"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00004318", "sets": ["323"]}, "author_link": ["2513", "2361", "2364"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-05-21", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "205", "bibliographicPageStart": "199", "bibliographicVolumeNumber": "339", "bibliographic_titles": [{"bibliographic_title": "Journal of Molecular Biology"}, {"bibliographic_title": "Journal of Molecular Biology", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "桑島, 邦博"}], "nameIdentifiers": [{"nameIdentifier": "2364", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000070091444", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "70091444", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70091444"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "There are currently two contradictory models for the kinetics of the ATP-induced GroEL allosteric transition occurring around 20 μM ATP. One model, proposed by Horovitz et al. demonstrates the existence of two parallel pathways for the allosteric transition and an abrupt ATP-dependent switch from one pathway to the other. The other model, which was proposed by the present authors, shows no need to assume the parallel pathways, and a combination of the transition-state theory and the Monod–Wyman–Changeux model of allostery can explain the kinetics as well as the equilibrium of the transition. The discrepancy appears to be due to whether we regard the transition as reversible or irreversible. Thus, here we have investigated the reversibility of the allosteric transition between 0 μM and 70 μM ATP by the use of a stopped-flow double-jump technique, which has allowed us to monitor the kinetics of the reverse reaction from the relaxed state at a high ATP concentration to the tense state at a low ATP concentration. The tryptophan fluorescence of a tryptophan-inserted variant of GroEL was used to follow the kinetics. As a result, the allosteric transition was shown to be a reversible process, supporting the validity of our model. We also show that the structural environment around the ATP-binding site of GroEL in the transition state is very similar to that in the relaxed state (Φ=0.9) by using a Φ value analysis in the kinetic Monod–Wyman–Changeux model, which is analogous to the mutational Φ value analysis in protein folding.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier "}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.jmb.2004.03.026"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.jmb.2004.03.026", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2004 Elsevier Ltd. "}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0022-2836 ", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "INOBE, Tomonao "}], "nameIdentifiers": [{"nameIdentifier": "2513", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KUWAJIMA, Kunihiro "}], "nameIdentifiers": [{"nameIdentifier": "2361", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Φ Value Analysis of an Allosteric Transition of GroEL based on a Single-pathway Model", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Φ Value Analysis of an Allosteric Transition of GroEL based on a Single-pathway Model"}, {"subitem_title": "Φ Value Analysis of an Allosteric Transition of GroEL based on a Single-pathway Model", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["323"], "permalink_uri": "https://ir.soken.ac.jp/records/4318", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-03-20"}, "publish_date": "2014-03-20", "publish_status": "0", "recid": "4318", "relation": {}, "relation_version_is_last": true, "title": ["Φ Value Analysis of an Allosteric Transition of GroEL based on a Single-pathway Model"], "weko_shared_id": 21}
  1. 010 学術雑誌論文
  2. 桑島, 邦博 / KUWAJIMA, Kunihiro

Φ Value Analysis of an Allosteric Transition of GroEL based on a Single-pathway Model

https://ir.soken.ac.jp/records/4318
https://ir.soken.ac.jp/records/4318
559500e2-b26a-4d3b-90e4-ae79113bedbc
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-03-20
タイトル
タイトル Φ Value Analysis of an Allosteric Transition of GroEL based on a Single-pathway Model
タイトル
言語 en
タイトル Φ Value Analysis of an Allosteric Transition of GroEL based on a Single-pathway Model
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 INOBE, Tomonao

× INOBE, Tomonao

WEKO 2513

INOBE, Tomonao

Search repository
KUWAJIMA, Kunihiro

× KUWAJIMA, Kunihiro

WEKO 2361

KUWAJIMA, Kunihiro

Search repository
著者別名 桑島, 邦博

× 桑島, 邦博

WEKO 2364
NRID 1000070091444
e-Rad 70091444

桑島, 邦博

Search repository
抄録
内容記述タイプ Abstract
内容記述 There are currently two contradictory models for the kinetics of the ATP-induced GroEL allosteric transition occurring around 20 μM ATP. One model, proposed by Horovitz et al. demonstrates the existence of two parallel pathways for the allosteric transition and an abrupt ATP-dependent switch from one pathway to the other. The other model, which was proposed by the present authors, shows no need to assume the parallel pathways, and a combination of the transition-state theory and the Monod–Wyman–Changeux model of allostery can explain the kinetics as well as the equilibrium of the transition. The discrepancy appears to be due to whether we regard the transition as reversible or irreversible. Thus, here we have investigated the reversibility of the allosteric transition between 0 μM and 70 μM ATP by the use of a stopped-flow double-jump technique, which has allowed us to monitor the kinetics of the reverse reaction from the relaxed state at a high ATP concentration to the tense state at a low ATP concentration. The tryptophan fluorescence of a tryptophan-inserted variant of GroEL was used to follow the kinetics. As a result, the allosteric transition was shown to be a reversible process, supporting the validity of our model. We also show that the structural environment around the ATP-binding site of GroEL in the transition state is very similar to that in the relaxed state (Φ=0.9) by using a Φ value analysis in the kinetic Monod–Wyman–Changeux model, which is analogous to the mutational Φ value analysis in protein folding.
書誌情報 Journal of Molecular Biology
en : Journal of Molecular Biology

巻 339, 号 1, p. 199-205, 発行日 2004-05-21
出版者
出版者 Elsevier
ISSN
収録物識別子タイプ ISSN
収録物識別子 0022-2836
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.jmb.2004.03.026
関連名称 10.1016/j.jmb.2004.03.026
権利
権利情報 © 2004 Elsevier Ltd.
戻る
0
views
See details
Views

Versions

Ver.1 2023-06-20 14:25:02.945541
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3