{"_buckets": {"deposit": "ff49045f-16d3-48d4-9aa8-b0846a03e52a"}, "_deposit": {"created_by": 21, "id": "4322", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "4322"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00004322", "sets": ["323"]}, "author_link": ["2361", "2364", "2523", "3119"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-10-19", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "53", "bibliographicPageEnd": "55769", "bibliographicPageStart": "55760", "bibliographicVolumeNumber": "279", "bibliographic_titles": [{"bibliographic_title": "Journal of Biological Chemistry"}, {"bibliographic_title": "Journal of Biological Chemistry", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "桑島, 邦博"}], "nameIdentifiers": [{"nameIdentifier": "2364", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000070091444", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "70091444", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70091444"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Hsp33, an Escherichia coli cytosolic chaperone, is inactive under normal conditions but becomes active upon oxidative stress. It was previously shown to dimerize upon activation in a concentration- and temperature-dependent manner. This dimer was thought to bind to aggregation-prone target proteins, preventing their aggregation. In the present study, we report small angle x-ray scattering (SAXS), steady state and time-resolved fluorescence, gel filtration, and glutaraldehyde cross-linking analysis of full-length Hsp33. Our circular dichroism and fluorescence results show that there are significant structural changes in oxidized Hsp33 at different temperatures. SAXS, gel filtration, and glutaraldehyde cross-linking results indicate, in addition to the dimers, the presence of oligomeric species. Oxidation in the presence of physiological salt concentration leads to significant increases in the oligomer population. Our results further show that under conditions that mimic the crowded milieu of the cytosol, oxidized Hsp33 exists predominantly as an oligomeric species. Interestingly, chaperone activity studies show that the oligomeric species is much more efficient compared with the dimers in preventing aggregation of target proteins. Taken together, these results indicate that in the cell, Hsp33 undergoes conformational and quaternary structural changes leading to the formation of oligomeric species in response to oxidative stress. Oligomeric Hsp33 thus might be physiologically relevant under oxidative stress. ", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Society for Biochemistry and Molecular Biology "}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1074/jbc.M406333200"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.1074/jbc.M406333200", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2014 by American Society for Biochemistry and Molecular Biology "}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0021-9258 ", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "AKHTAR, Mohd. Waseem "}], "nameIdentifiers": [{"nameIdentifier": "2523", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KUWAJIMA, Kunihiro "}], "nameIdentifiers": [{"nameIdentifier": "2361", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "et, al."}], "nameIdentifiers": [{"nameIdentifier": "3119", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Oligomeric Hsp33 with Enhanced Chaperone Activity", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Oligomeric Hsp33 with Enhanced Chaperone Activity"}, {"subitem_title": "Oligomeric Hsp33 with Enhanced Chaperone Activity", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["323"], "permalink_uri": "https://ir.soken.ac.jp/records/4322", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-03-20"}, "publish_date": "2014-03-20", "publish_status": "0", "recid": "4322", "relation": {}, "relation_version_is_last": true, "title": ["Oligomeric Hsp33 with Enhanced Chaperone Activity"], "weko_shared_id": 21}