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  1. 010 学術雑誌論文
  2. 桑島, 邦博 / KUWAJIMA, Kunihiro

Oligomeric Hsp33 with Enhanced Chaperone Activity

https://ir.soken.ac.jp/records/4322
https://ir.soken.ac.jp/records/4322
1a51038c-de53-4d23-8188-de3c8a8ed054
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-03-20
タイトル
タイトル Oligomeric Hsp33 with Enhanced Chaperone Activity
タイトル
タイトル Oligomeric Hsp33 with Enhanced Chaperone Activity
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 AKHTAR, Mohd. Waseem

× AKHTAR, Mohd. Waseem

AKHTAR, Mohd. Waseem

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KUWAJIMA, Kunihiro

× KUWAJIMA, Kunihiro

KUWAJIMA, Kunihiro

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et, al.

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et, al.

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著者別名 桑島, 邦博

× 桑島, 邦博

桑島, 邦博

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抄録
内容記述タイプ Abstract
内容記述 Hsp33, an Escherichia coli cytosolic chaperone, is inactive under normal conditions but becomes active upon oxidative stress. It was previously shown to dimerize upon activation in a concentration- and temperature-dependent manner. This dimer was thought to bind to aggregation-prone target proteins, preventing their aggregation. In the present study, we report small angle x-ray scattering (SAXS), steady state and time-resolved fluorescence, gel filtration, and glutaraldehyde cross-linking analysis of full-length Hsp33. Our circular dichroism and fluorescence results show that there are significant structural changes in oxidized Hsp33 at different temperatures. SAXS, gel filtration, and glutaraldehyde cross-linking results indicate, in addition to the dimers, the presence of oligomeric species. Oxidation in the presence of physiological salt concentration leads to significant increases in the oligomer population. Our results further show that under conditions that mimic the crowded milieu of the cytosol, oxidized Hsp33 exists predominantly as an oligomeric species. Interestingly, chaperone activity studies show that the oligomeric species is much more efficient compared with the dimers in preventing aggregation of target proteins. Taken together, these results indicate that in the cell, Hsp33 undergoes conformational and quaternary structural changes leading to the formation of oligomeric species in response to oxidative stress. Oligomeric Hsp33 thus might be physiologically relevant under oxidative stress.
書誌情報 Journal of Biological Chemistry
en : Journal of Biological Chemistry

巻 279, 号 53, p. 55760-55769, 発行日 2004-10-19
出版者
出版者 American Society for Biochemistry and Molecular Biology
ISSN
収録物識別子タイプ ISSN
収録物識別子 0021-9258
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.1074/jbc.M406333200
関連名称 10.1074/jbc.M406333200
権利
権利情報 © 2014 by American Society for Biochemistry and Molecular Biology
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