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Identical sets of methylated and nonmethylated genes in Ciona intestinalis sperm and muscle cells
https://ir.soken.ac.jp/records/4640
https://ir.soken.ac.jp/records/46401024d29f-4c14-424a-9204-d017ba28d948
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
1756-8935-6-38 (843.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-06-10 | |||||
| タイトル | ||||||
| タイトル | Identical sets of methylated and nonmethylated genes in Ciona intestinalis sperm and muscle cells | |||||
| タイトル | ||||||
| タイトル | Identical sets of methylated and nonmethylated genes in Ciona intestinalis sperm and muscle cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
SUZUKI, Miho M.
× SUZUKI, Miho M.× TAKUNO, Shohei× et, al. |
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| 著者別名 |
宅野, 将平
× 宅野, 将平 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background The discovery of gene body methylation, which refers to DNA methylation within gene coding region, suggests an as yet unknown role of DNA methylation at actively transcribed genes. In invertebrates, gene bodies are the primary targets of DNA methylation, and only a subset of expressed genes is modified. Results Here we investigate the tissue variability of both the global levels and distribution of 5-methylcytosine (5mC) in the sea squirt Ciona intestinalis. We find that global 5mC content of early developmental embryos is high, but is strikingly reduced in body wall tissues. We chose sperm and adult muscle cells, with high and reduced levels of global 5mC respectively, for genome-wide analysis of 5mC targets. By means of CXXC-affinity purification followed by deep sequencing (CAP-seq), and genome-wide bisulfite sequencing (BS-seq), we designated body-methylated and unmethylated genes in each tissue. Surprisingly, body-methylated and unmethylated gene groups are identical in the sperm and muscle cells. Our analysis of microarray expression data shows that gene body methylation is associated with broad expression throughout development. Moreover, transgenic analysis reveals contrasting gene body methylation at an identical gene-promoter combination when integrated at different genomic sites. Conclusions We conclude that gene body methylation is not a direct regulator of tissue specific gene expression in C. intestinalis. Our findings reveal constant targeting of gene body methylation irrespective of cell type, and they emphasize a correlation between gene body methylation and ubiquitously expressed genes. Our transgenic experiments suggest that the promoter does not determine the methylation status of the associated gene body. |
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| 書誌情報 |
Epigenetics & Chromatin en : Epigenetics & Chromatin 巻 6, p. 38, 発行日 2013-11 |
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| 出版者 | ||||||
| 出版者 | BioMed Central | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1756-8935 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1186/1756-8935-6-38 | |||||
| 関連名称 | 10.1186/1756-8935-6-38 | |||||
| 権利 | ||||||
| 権利情報 | © 2013 Suzuki et al. | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.biomedcentral.com/about/license | |||||
| 関連名称 | Copyright | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
