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(1991)\u003cbr /\u003e \u003cu\u003eMol.\u003c/u\u003e \u003cu\u003eGen.\u003c/u\u003e \u003cu\u003eGenet.\u003c/u\u003e 230, 17-24). The relative levels of these three holoenzyme\u003cbr /\u003e forms changed depending on the phase of cell growth. In the \u003cu\u003ein\u003c/u\u003e \u003cu\u003evitro\u003c/u\u003e mixed\u003cbr /\u003e transcription assay using 33 different \u003cu\u003eE.\u003c/u\u003e \u003cu\u003ecoli\u003c/u\u003e promoters, one of the\u003cbr /\u003e stationary-phase RNA polymerase, S1, showed promoter recognition properties\u003cbr /\u003e which are significantly different from that of holoenzyme from exponentially\u003cbr /\u003e growing cells. \u003cbr /\u003e Enzyme reconstitution experiments showed that the altered promoter\u003cbr /\u003e selectivity is due to alteration in core enzyme. After a variety of attempts\u003cbr /\u003e to achieve \u003cu\u003ein\u003c/u\u003e \u003cu\u003evitro\u003c/u\u003e interconversion between the exponential and the stationary\u003cbr /\u003e phase RNA polymerases, S1-form enzyme was found to be converted \u003cu\u003ein\u003c/u\u003e \u003cu\u003evitro\u003c/u\u003e into\u003cbr /\u003e such an enzyme as the log-phase form, following incubation with nucleotides or\u003cbr /\u003e pyrophosphate (Ozaki, M. \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., (1991) \u003cu\u003eNucleic\u003c/u\u003e \u003cu\u003eAcids\u003c/u\u003e \u003cu\u003eRes.\u003c/u\u003e in press). The\u003cbr /\u003e conversion was indicated by not only the shift of elution position from a\u003cbr /\u003e phosphocellulose column but also the change in the promoter selectivity. Using\u003cbr /\u003e polyphosphate kinase (PPK) which polymerizes the terminal phosphate of ATP to\u003cbr /\u003e a long chain polyphosphate in a freely reversible reaction, polyphosphate was\u003cbr /\u003e detected in the stationary-phase RNA polymerase (Ozakl, M. \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., in\u003cbr /\u003e preparation). These results altogether lead to the possibility that RNA\u003cbr /\u003e polymerase is converted into the stationary-phase form by binding\u003cbr /\u003e polyphosphate. I propose that the modulation of RNA polymerase by\u003cbr /\u003e polyphosphate plays a role in the global switch of gene transcription during\u003cbr /\u003e the growth transition of \u003cu\u003eE.\u003c/u\u003e \u003cu\u003ecoli\u003c/u\u003e to stationary phase.", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第18号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "18 遺伝学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "1991"}]}, "item_1_text_20": {"attribute_name": "業務メモ", "attribute_value_mlt": [{"subitem_text_value": "(2018年2月19日)本籍など個人情報の記載がある旧要旨・審査要旨を個人情報のない新しいものに差し替えた。承諾書等未確認。要確認該当項目修正のこと。"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "OZAKI, Miwako", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9809", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲18_要旨.pdf", "filesize": [{"value": "189.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 189500.0, "url": {"label": "要旨・審査要旨 / Abstract, Screening Result", "url": "https://ir.soken.ac.jp/record/879/files/甲18_要旨.pdf"}, "version_id": "9aea7298-fb0e-4523-9fa0-b838e45898e2"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli"}, {"subitem_title": "Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["20"], "permalink_uri": "https://ir.soken.ac.jp/records/879", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "879", "relation": {}, "relation_version_is_last": true, "title": ["Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli"], "weko_shared_id": 1}
Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli
https://ir.soken.ac.jp/records/879
https://ir.soken.ac.jp/records/87980b95296-92cf-4367-b010-335c3af57c08
名前 / ファイル | ライセンス | アクション |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2010-02-22 | |||||
タイトル | ||||||
タイトル | Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
資源タイプ | thesis | |||||
著者名 |
尾崎, 美和子
× 尾崎, 美和子 |
|||||
フリガナ |
オザキ, ミワコ
× オザキ, ミワコ |
|||||
著者 |
OZAKI, Miwako
× OZAKI, Miwako |
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学位授与機関 | ||||||
学位授与機関名 | 総合研究大学院大学 | |||||
学位名 | ||||||
学位名 | 博士(理学) | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 総研大甲第18号 | |||||
研究科 | ||||||
値 | 生命科学研究科 | |||||
専攻 | ||||||
値 | 18 遺伝学専攻 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 1992-03-16 | |||||
学位授与年度 | ||||||
1991 | ||||||
要旨 | ||||||
内容記述タイプ | Other | |||||
内容記述 | During the growth phase transition of <u>Escherichia</u> <u>coli</u> from exponential<br /> growth to stationary phase, the pre-existing RNA polymerase was found to be<br /> converted into at least three different holoenzyme forms, which could be<br /> isolated by phosphocellulose column chromatography (Ozaki, M., <u>et</u> <u>al</u>. (1991)<br /> <u>Mol.</u> <u>Gen.</u> <u>Genet.</u> 230, 17-24). The relative levels of these three holoenzyme<br /> forms changed depending on the phase of cell growth. In the <u>in</u> <u>vitro</u> mixed<br /> transcription assay using 33 different <u>E.</u> <u>coli</u> promoters, one of the<br /> stationary-phase RNA polymerase, S1, showed promoter recognition properties<br /> which are significantly different from that of holoenzyme from exponentially<br /> growing cells. <br /> Enzyme reconstitution experiments showed that the altered promoter<br /> selectivity is due to alteration in core enzyme. After a variety of attempts<br /> to achieve <u>in</u> <u>vitro</u> interconversion between the exponential and the stationary<br /> phase RNA polymerases, S1-form enzyme was found to be converted <u>in</u> <u>vitro</u> into<br /> such an enzyme as the log-phase form, following incubation with nucleotides or<br /> pyrophosphate (Ozaki, M. <u>et</u> <u>al</u>., (1991) <u>Nucleic</u> <u>Acids</u> <u>Res.</u> in press). The<br /> conversion was indicated by not only the shift of elution position from a<br /> phosphocellulose column but also the change in the promoter selectivity. Using<br /> polyphosphate kinase (PPK) which polymerizes the terminal phosphate of ATP to<br /> a long chain polyphosphate in a freely reversible reaction, polyphosphate was<br /> detected in the stationary-phase RNA polymerase (Ozakl, M. <u>et</u> <u>al</u>., in<br /> preparation). These results altogether lead to the possibility that RNA<br /> polymerase is converted into the stationary-phase form by binding<br /> polyphosphate. I propose that the modulation of RNA polymerase by<br /> polyphosphate plays a role in the global switch of gene transcription during<br /> the growth transition of <u>E.</u> <u>coli</u> to stationary phase. | |||||
所蔵 | ||||||
値 | 有 |