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Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli
https://ir.soken.ac.jp/records/879
https://ir.soken.ac.jp/records/87980b95296-92cf-4367-b010-335c3af57c08
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
要旨・審査要旨 / Abstract, Screening Result (189.5 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-02-22 | |||||
| タイトル | ||||||
| タイトル | Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli | |||||
| タイトル | ||||||
| タイトル | Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 著者名 |
尾崎, 美和子
× 尾崎, 美和子 |
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| フリガナ |
オザキ, ミワコ
× オザキ, ミワコ |
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| 著者 |
OZAKI, Miwako
× OZAKI, Miwako |
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| 学位授与機関 | ||||||
| 学位授与機関名 | 総合研究大学院大学 | |||||
| 学位名 | ||||||
| 学位名 | 博士（理学） | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 総研大甲第18号 | |||||
| 研究科 | ||||||
| 値 | 生命科学研究科 | |||||
| 専攻 | ||||||
| 値 | 18 遺伝学専攻 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 1992-03-16 | |||||
| 学位授与年度 | ||||||
| 値 | 1991 | |||||
| 要旨 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | During the growth phase transition of <u>Escherichia</u> <u>coli</u> from exponential<br /> growth to stationary phase, the pre-existing RNA polymerase was found to be<br /> converted into at least three different holoenzyme forms, which could be<br /> isolated by phosphocellulose column chromatography (Ozaki, M., <u>et</u> <u>al</u>. (1991)<br /> <u>Mol.</u> <u>Gen.</u> <u>Genet.</u> 230, 17-24). The relative levels of these three holoenzyme<br /> forms changed depending on the phase of cell growth. In the <u>in</u> <u>vitro</u> mixed<br /> transcription assay using 33 different <u>E.</u> <u>coli</u> promoters, one of the<br /> stationary-phase RNA polymerase, S1, showed promoter recognition properties<br /> which are significantly different from that of holoenzyme from exponentially<br /> growing cells. <br /> Enzyme reconstitution experiments showed that the altered promoter<br /> selectivity is due to alteration in core enzyme. After a variety of attempts<br /> to achieve <u>in</u> <u>vitro</u> interconversion between the exponential and the stationary<br /> phase RNA polymerases, S1-form enzyme was found to be converted <u>in</u> <u>vitro</u> into<br /> such an enzyme as the log-phase form, following incubation with nucleotides or<br /> pyrophosphate (Ozaki, M. <u>et</u> <u>al</u>., (1991) <u>Nucleic</u> <u>Acids</u> <u>Res.</u> in press). The<br /> conversion was indicated by not only the shift of elution position from a<br /> phosphocellulose column but also the change in the promoter selectivity. Using<br /> polyphosphate kinase (PPK) which polymerizes the terminal phosphate of ATP to<br /> a long chain polyphosphate in a freely reversible reaction, polyphosphate was<br /> detected in the stationary-phase RNA polymerase (Ozakl, M. <u>et</u> <u>al</u>., in<br /> preparation). These results altogether lead to the possibility that RNA<br /> polymerase is converted into the stationary-phase form by binding<br /> polyphosphate. I propose that the modulation of RNA polymerase by<br /> polyphosphate plays a role in the global switch of gene transcription during<br /> the growth transition of <u>E.</u> <u>coli</u> to stationary phase. | |||||
| 所蔵 | ||||||
| 値 | 有 | |||||
