{"_buckets": {"deposit": "996f8bba-df1e-4880-9261-c43b40486b4d"}, "_deposit": {"created_by": 1, "id": "879", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "879"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00000879", "sets": ["20"]}, "author_link": ["9809", "9808", "9807"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1992-03-16", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "尾崎, 美和子"}], "nameIdentifiers": [{"nameIdentifier": "9807", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "オザキ, ミワコ"}], "nameIdentifiers": [{"nameIdentifier": "9808", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "1992-03-16"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "1992018", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "　　During the growth phase transition of \u003cu\u003eEscherichia\u003c/u\u003e \u003cu\u003ecoli\u003c/u\u003e from exponential\u003cbr /\u003e growth to stationary phase, the pre-existing RNA polymerase was found to be\u003cbr /\u003e converted into at least three different holoenzyme forms, which could be\u003cbr /\u003e isolated by phosphocellulose column chromatography (Ozaki, M., \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e. (1991)\u003cbr /\u003e \u003cu\u003eMol.\u003c/u\u003e \u003cu\u003eGen.\u003c/u\u003e \u003cu\u003eGenet.\u003c/u\u003e 230, 17-24).　The relative levels of these three holoenzyme\u003cbr /\u003e forms changed depending on the phase of cell growth. In the \u003cu\u003ein\u003c/u\u003e \u003cu\u003evitro\u003c/u\u003e mixed\u003cbr /\u003e transcription assay using 33 different \u003cu\u003eE.\u003c/u\u003e \u003cu\u003ecoli\u003c/u\u003e promoters, one of the\u003cbr /\u003e stationary-phase RNA polymerase, S1, showed promoter recognition properties\u003cbr /\u003e which are significantly different from that of holoenzyme from exponentially\u003cbr /\u003e growing cells. \u003cbr /\u003e　　Enzyme reconstitution experiments showed that the altered promoter\u003cbr /\u003e selectivity is due to alteration in core enzyme. After a variety of attempts\u003cbr /\u003e to achieve \u003cu\u003ein\u003c/u\u003e \u003cu\u003evitro\u003c/u\u003e interconversion between the exponential and the stationary\u003cbr /\u003e phase RNA polymerases, S1-form enzyme was found to be converted \u003cu\u003ein\u003c/u\u003e \u003cu\u003evitro\u003c/u\u003e into\u003cbr /\u003e such an enzyme as the log-phase form, following incubation with nucleotides or\u003cbr /\u003e pyrophosphate (Ozaki, M. \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., (1991) \u003cu\u003eNucleic\u003c/u\u003e \u003cu\u003eAcids\u003c/u\u003e \u003cu\u003eRes.\u003c/u\u003e in press). The\u003cbr /\u003e conversion was indicated by not only the shift of elution position from a\u003cbr /\u003e phosphocellulose column but also the change in the promoter selectivity. Using\u003cbr /\u003e polyphosphate kinase (PPK) which polymerizes the terminal phosphate of ATP to\u003cbr /\u003e a long chain polyphosphate in a freely reversible reaction, polyphosphate was\u003cbr /\u003e detected in the stationary-phase RNA polymerase (Ozakl, M. \u003cu\u003eet\u003c/u\u003e \u003cu\u003eal\u003c/u\u003e., in\u003cbr /\u003e preparation). These results altogether lead to the possibility that RNA\u003cbr /\u003e polymerase is converted into the stationary-phase form by binding\u003cbr /\u003e polyphosphate. I propose that the modulation of RNA polymerase by\u003cbr /\u003e polyphosphate plays a role in the global switch of gene transcription during\u003cbr /\u003e the growth transition of \u003cu\u003eE.\u003c/u\u003e \u003cu\u003ecoli\u003c/u\u003e to stationary phase.", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第18号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "18 遺伝学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "1991"}]}, "item_1_text_20": {"attribute_name": "業務メモ", "attribute_value_mlt": [{"subitem_text_value": "（2018年2月19日）本籍など個人情報の記載がある旧要旨・審査要旨を個人情報のない新しいものに差し替えた。承諾書等未確認。要確認該当項目修正のこと。"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "OZAKI, Miwako", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9809", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲18_要旨.pdf", "filesize": [{"value": "189.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 189500.0, "url": {"label": "要旨・審査要旨 / Abstract, Screening Result", "url": "https://ir.soken.ac.jp/record/879/files/甲18_要旨.pdf"}, "version_id": "9aea7298-fb0e-4523-9fa0-b838e45898e2"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli"}, {"subitem_title": "Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["20"], "permalink_uri": "https://ir.soken.ac.jp/records/879", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "879", "relation": {}, "relation_version_is_last": true, "title": ["Structural and Functional Modulations of RNApolymerase During Growth Phase Transition ofEscherichia coli"], "weko_shared_id": 1}