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It binds to a 9 base pairs sequence in the upstream\u003cbr /\u003e regulatory region of the \u003ci\u003efushi tarazu (ftz)\u003c/i\u003e gene. Binding site-dependent\u003cbr /\u003e expression of the \u003ci\u003eftz-lacZ\u003c/i\u003e fusion genes in transformed embryos showed\u003cbr /\u003e that FTZ-F1 is a positive regulator of the \u003ci\u003eftz\u003c/i\u003e gene. A posterior silk gland\u003cbr /\u003e extract from the silkworm \u003ci\u003eBombyx mori\u003c/i\u003e contains a factor termed\u003cbr /\u003e BmFTZ-F1 which recognizes the same DNA sequence as FTZ-F1 and\u003cbr /\u003e has many biochemical characters similar to FTZ-F1. Molecular cloning\u003cbr /\u003e of cDNAs for FTZ-F1 and BmFTZ-F1 revealed that they are members of\u003cbr /\u003e the steroid hormone receptor superfamily and share homologies in the\u003cbr /\u003e DNA-binding and the putative ligand-binding domains. \u003cbr /\u003e Using the \u003ci\u003ein vitro\u003c/i\u003e transcription systems from posterior silk gland\u003cbr /\u003e cells, I found that BmFTZ-F1 can activate transcription of the \u003ci\u003eftz\u003c/i\u003e gene\u003cbr /\u003e in a FTZ-F1-binding site dependent manner. To elucidate the mechanism\u003cbr /\u003e of transactivation by BmFTZ-F1, I used \u003ci\u003ein vitro\u003c/i\u003e transcription systems\u003cbr /\u003e from HeLa cells. Because the HeLa system does not contain a FTZ-F1 like\u003cbr /\u003e activity, it serves as a recipient in complementation assay for active\u003cbr /\u003e components derived from the posterior silk gland extract. The results of\u003cbr /\u003e these analyses suggest that two proteins (18kDa and 22kDa polypeptides)\u003cbr /\u003e termed MBF (\u003ci\u003em\u003c/i\u003eediator of BmFTZ-F1) 1 and MBF2, respectively, that\u003cbr /\u003e form a heterodimer and mediate activiation of \u003ci\u003ein vitro\u003c/i\u003e transcription\u003cbr /\u003e from the \u003ci\u003eftz\u003c/i\u003e gene promotor by BmFTZ-F1. Neither MBF1 nor MBF2\u003cbr /\u003e binds to DNA. MBF1 interacts with BmFTZ-F1 and stabilizes the\u003cbr /\u003e BmFTZ-F1・DNA complex. MBF1 also makes a direct contact with\u003cbr /\u003e TATA-binding protein (TBP). Both MBF1 and MBF2 are necessary to\u003cbr /\u003e form a complex between BmFTZ-F1 and TBP. I propose a model in\u003cbr /\u003e which MBF1 and MBF2 form a bridge between BmFTZ-F1 and TBP,\u003cbr /\u003e and mediate transactivation by stabilizing the protein・DNA interactions.\u003cbr /\u003e This is the first isolation of mediators capable of modulating transactivator\u003cbr /\u003e function directly.", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第89号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "18 遺伝学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "1993"}]}, "item_1_text_20": {"attribute_name": "業務メモ", "attribute_value_mlt": [{"subitem_text_value": "(2018年2月19日)本籍など個人情報の記載がある旧要旨・審査要旨を個人情報のない新しいものに差し替えた。承諾書等未確認。要確認該当項目修正のこと。"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "LI, Feng-Qian", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9857", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲89_要旨.pdf", "filesize": [{"value": "195.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 195300.0, "url": {"label": "要旨・審査要旨 / Abstract, Screening Result", "url": "https://ir.soken.ac.jp/record/895/files/甲89_要旨.pdf"}, "version_id": "5b3fb39d-76d9-47d1-ab3a-7001d3fbea75"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "甲89_本文.pdf", "filesize": [{"value": "1.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1800000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/895/files/甲89_本文.pdf"}, "version_id": "4ea206c4-b351-4313-8869-6a07a3c6026c"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1"}, {"subitem_title": "Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["20"], "permalink_uri": "https://ir.soken.ac.jp/records/895", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "895", "relation": {}, "relation_version_is_last": true, "title": ["Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1"], "weko_shared_id": 1}
Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1
https://ir.soken.ac.jp/records/895
https://ir.soken.ac.jp/records/8956bc816cf-4586-47ee-9ae2-1766dcfe53c3
名前 / ファイル | ライセンス | アクション |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2010-02-22 | |||||
タイトル | ||||||
タイトル | Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
資源タイプ | thesis | |||||
著者名 |
李, 豊倩
× 李, 豊倩 |
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フリガナ |
リ, ホウセイ
× リ, ホウセイ |
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著者 |
LI, Feng-Qian
× LI, Feng-Qian |
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学位授与機関 | ||||||
学位授与機関名 | 総合研究大学院大学 | |||||
学位名 | ||||||
学位名 | 博士(理学) | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 総研大甲第89号 | |||||
研究科 | ||||||
値 | 生命科学研究科 | |||||
専攻 | ||||||
値 | 18 遺伝学専攻 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 1994-03-24 | |||||
学位授与年度 | ||||||
1993 | ||||||
要旨 | ||||||
内容記述タイプ | Other | |||||
内容記述 | FTZ-FI has been identified as a sequence specific DNA-binding<br /> protein in <i>Drosophila</i>. It binds to a 9 base pairs sequence in the upstream<br /> regulatory region of the <i>fushi tarazu (ftz)</i> gene. Binding site-dependent<br /> expression of the <i>ftz-lacZ</i> fusion genes in transformed embryos showed<br /> that FTZ-F1 is a positive regulator of the <i>ftz</i> gene. A posterior silk gland<br /> extract from the silkworm <i>Bombyx mori</i> contains a factor termed<br /> BmFTZ-F1 which recognizes the same DNA sequence as FTZ-F1 and<br /> has many biochemical characters similar to FTZ-F1. Molecular cloning<br /> of cDNAs for FTZ-F1 and BmFTZ-F1 revealed that they are members of<br /> the steroid hormone receptor superfamily and share homologies in the<br /> DNA-binding and the putative ligand-binding domains. <br /> Using the <i>in vitro</i> transcription systems from posterior silk gland<br /> cells, I found that BmFTZ-F1 can activate transcription of the <i>ftz</i> gene<br /> in a FTZ-F1-binding site dependent manner. To elucidate the mechanism<br /> of transactivation by BmFTZ-F1, I used <i>in vitro</i> transcription systems<br /> from HeLa cells. Because the HeLa system does not contain a FTZ-F1 like<br /> activity, it serves as a recipient in complementation assay for active<br /> components derived from the posterior silk gland extract. The results of<br /> these analyses suggest that two proteins (18kDa and 22kDa polypeptides)<br /> termed MBF (<i>m</i>ediator of BmFTZ-F1) 1 and MBF2, respectively, that<br /> form a heterodimer and mediate activiation of <i>in vitro</i> transcription<br /> from the <i>ftz</i> gene promotor by BmFTZ-F1. Neither MBF1 nor MBF2<br /> binds to DNA. MBF1 interacts with BmFTZ-F1 and stabilizes the<br /> BmFTZ-F1・DNA complex. MBF1 also makes a direct contact with<br /> TATA-binding protein (TBP). Both MBF1 and MBF2 are necessary to<br /> form a complex between BmFTZ-F1 and TBP. I propose a model in<br /> which MBF1 and MBF2 form a bridge between BmFTZ-F1 and TBP,<br /> and mediate transactivation by stabilizing the protein・DNA interactions.<br /> This is the first isolation of mediators capable of modulating transactivator<br /> function directly. | |||||
所蔵 | ||||||
値 | 有 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |