{"_buckets": {"deposit": "e3efc185-9012-4feb-812f-6938c5265116"}, "_deposit": {"created_by": 1, "id": "895", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "895"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00000895", "sets": ["20"]}, "author_link": ["9856", "9855", "9857"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1994-03-24", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "李, 豊倩"}], "nameIdentifiers": [{"nameIdentifier": "9855", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "リ, ホウセイ"}], "nameIdentifiers": [{"nameIdentifier": "9856", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "1994-03-24"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "1994025", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "　　　FTZ-FI has been identified as a sequence specific DNA-binding\u003cbr /\u003e protein in \u003ci\u003eDrosophila\u003c/i\u003e. It binds to a 9 base pairs sequence in the upstream\u003cbr /\u003e regulatory region of the \u003ci\u003efushi tarazu (ftz)\u003c/i\u003e gene. Binding site-dependent\u003cbr /\u003e expression of the \u003ci\u003eftz-lacZ\u003c/i\u003e fusion genes in transformed embryos showed\u003cbr /\u003e that FTZ-F1 is a positive regulator of the \u003ci\u003eftz\u003c/i\u003e gene. A posterior silk gland\u003cbr /\u003e extract from the silkworm \u003ci\u003eBombyx mori\u003c/i\u003e contains a factor termed\u003cbr /\u003e BmFTZ-F1 which recognizes the same DNA sequence as FTZ-F1 and\u003cbr /\u003e has many biochemical characters similar to FTZ-F1. Molecular cloning\u003cbr /\u003e of cDNAs for FTZ-F1 and BmFTZ-F1 revealed that they are members of\u003cbr /\u003e the steroid hormone receptor superfamily and share homologies in the\u003cbr /\u003e DNA-binding and the putative ligand-binding domains. \u003cbr /\u003e　　　Using the \u003ci\u003ein vitro\u003c/i\u003e transcription systems from posterior silk gland\u003cbr /\u003e cells, I found that BmFTZ-F1 can activate transcription of the \u003ci\u003eftz\u003c/i\u003e gene\u003cbr /\u003e in a FTZ-F1-binding site dependent manner. To elucidate the mechanism\u003cbr /\u003e of transactivation by BmFTZ-F1, I used \u003ci\u003ein vitro\u003c/i\u003e transcription systems\u003cbr /\u003e from HeLa cells. Because the HeLa system does not contain a FTZ-F1 like\u003cbr /\u003e activity, it serves as a recipient in complementation assay for active\u003cbr /\u003e components derived from the posterior silk gland extract. The results of\u003cbr /\u003e these analyses suggest that two proteins (18kDa and 22kDa polypeptides)\u003cbr /\u003e termed MBF (\u003ci\u003em\u003c/i\u003eediator of BmFTZ-F1) 1 and MBF2, respectively, that\u003cbr /\u003e form a heterodimer and mediate activiation of \u003ci\u003ein vitro\u003c/i\u003e transcription\u003cbr /\u003e from the \u003ci\u003eftz\u003c/i\u003e gene promotor by BmFTZ-F1. Neither MBF1 nor MBF2\u003cbr /\u003e binds to DNA. MBF1 interacts with BmFTZ-F1 and stabilizes the\u003cbr /\u003e BmFTZ-F1･DNA complex. MBF1 also makes a direct contact with\u003cbr /\u003e TATA-binding protein (TBP). Both MBF1 and MBF2 are necessary to\u003cbr /\u003e form a complex between BmFTZ-F1 and TBP. I propose a model in\u003cbr /\u003e which MBF1 and MBF2 form a bridge between BmFTZ-F1 and TBP,\u003cbr /\u003e and mediate transactivation by stabilizing the protein･DNA interactions.\u003cbr /\u003e This is the first isolation of mediators capable of modulating transactivator\u003cbr /\u003e function directly.", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第89号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "18 遺伝学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "1993"}]}, "item_1_text_20": {"attribute_name": "業務メモ", "attribute_value_mlt": [{"subitem_text_value": "（2018年2月19日）本籍など個人情報の記載がある旧要旨・審査要旨を個人情報のない新しいものに差し替えた。承諾書等未確認。要確認該当項目修正のこと。"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "LI, Feng-Qian", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9857", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲89_要旨.pdf", "filesize": [{"value": "195.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 195300.0, "url": {"label": "要旨・審査要旨 / Abstract, Screening Result", "url": "https://ir.soken.ac.jp/record/895/files/甲89_要旨.pdf"}, "version_id": "5b3fb39d-76d9-47d1-ab3a-7001d3fbea75"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "甲89_本文.pdf", "filesize": [{"value": "1.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1800000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/895/files/甲89_本文.pdf"}, "version_id": "4ea206c4-b351-4313-8869-6a07a3c6026c"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1"}, {"subitem_title": "Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["20"], "permalink_uri": "https://ir.soken.ac.jp/records/895", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "895", "relation": {}, "relation_version_is_last": true, "title": ["Mediators for Activation of fushi tarazu Gene Transcription by BmFTZ-F1"], "weko_shared_id": 1}